Screening and evaluation of lactic acid bacteria with probiotic potential from local Holstein raw milk
There are massive bacteria in the raw milk, especially the lactic acid bacteria (LABs), which have been considered probiotics in humans and animals for a long time. Novel probiotics are still urgently needed because of the rapid development of the probiotic industry. To obtain new LABs with high pro...
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Published in | Frontiers in microbiology Vol. 13; p. 918774 |
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Main Authors | , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
01.08.2022
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Subjects | |
Online Access | Get full text |
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Summary: | There are massive bacteria in the raw milk, especially the lactic acid bacteria (LABs), which have been considered probiotics in humans and animals for a long time. Novel probiotics are still urgently needed because of the rapid development of the probiotic industry. To obtain new LABs with high probiotic potential, we obtained 26 LAB isolates, named L1 ~ L26, from local Holstein raw milk collected from a farm whose milk had never been used for LAB isolation. We identified them at the species level by biochemical and 16S rDNA sequencing methods. Their antagonistic activities against four target pathogens (
Escherichia coli
ATCC 25922,
Staphylococcus aureus
ATCC 25923,
Pseudomonas aeruginosa
PAO1, and
Salmonella enterica
H9812), co-aggregative ability with these target pathogens, survivability in the simulated gastrointestinal tract conditions and phenol, auto-aggregation and hydrophobicity, hemolytic activity, and antibiotic susceptibility, were evaluated
in vitro
. Five
Lactiplantibacillus plantarum
isolates (L5, L14, L17, L19, and L20) showed more promising probiotic potential than others. Specifically, these five isolates conglutinated with and inhibited all the target pathogens, and survived in the simulated gastric juice (92.55 ~ 99.69%), intestinal juice (76.18 ~ 83.39%), and 0.4% phenol (76.95 ~ 88.91%); possessed considerable auto-aggregation (83.91 ~ 90.33% at 24 h) and hydrophobicity (79.32 ~ 92.70%); and were non-hemolytic, sensitive to kinds of common antimicrobials. Our findings demonstrated that these five isolates could be preliminarily determined as probiotic candidates because they have better probiotic potential than those previously reported. Again, this study highlighted the potential of raw milk for probiotic isolating and screening and provided the probiotic industry with five new LAB candidates. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Specialty section: This article was submitted to Food Microbiology, a section of the journal Frontiers in Microbiology Edited by: Chong Zhang, Nanjing Agricultural University, China These authors have contributed equally to this work Reviewed by: Muhammad Bilal Sadiq, Forman Christian College, Pakistan; Xiangzhen Shen, Nanjing Agricultural University, China; Kang Yong, Gansu Agricultural University, China; Hualei Wang, Jilin University, China |
ISSN: | 1664-302X 1664-302X |
DOI: | 10.3389/fmicb.2022.918774 |