Phenotyping the haemostatic system by thrombography—potential for the estimation of thrombotic risk

• Published in: Thrombosis research Vol. 114; no. 5; pp. 539 - 545
• Main Authors: Regnault, Véronique, Hemker, H.Coenraad, Wahl, Denis, Lecompte, Thomas
• Format: Journal Article Conference Proceeding
• Language: English
• Published: New York, NY Elsevier Ltd 2004; Elsevier Science
• Subjects: Anticoagulants - pharmacology; Antiphospholipid antibodies; Biological and medical sciences; Blood and lymphatic vessels; Blood Coagulation Disorders - diagnosis; Blood Platelets - metabolism; Calibration; Cardiology. Vascular system; Coronary heart disease; Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous; Fluorometry - methods; Fundamental and applied biological sciences. Psychology; Heart; Hemostasis; Humans; Lymphatic system; Medical sciences; Phenotype; Plasma - immunology; Protein C; Protein C - chemistry; Protein C - pharmacology; Recombinant Proteins - chemistry; Risk; Software; Specimen Handling; Thrombin course; Thrombomodulin - chemistry; Thrombophilia; Thromboplastin - pharmacology; Thrombosis; Thrombosis - pathology; Time Factors; Vertebrates: cardiovascular system; Thrombophilia; Protein C; Thrombin course; Antiphospholipid antibodies; Thrombosis; Antiphospholipid antibody syndrome; Serine endopeptidases; Hemostatic; Enzyme; Estimation; Thrombophitia; Autoimmune disease; Cardiovascular disease; Thrombin; Hemopathy; Vascular disease; Peptidases; Platelet; Risk factor; Hydrolases
• ISSN: 0049-3848; 1879-2472
• DOI: 10.1016/j.thromres.2004.06.017

The aim of this paper is to review the thrombogram and its use for phenotyping the haemostatic system. The thrombogram can be readily obtained through Calibrated Automated Thrombography (CAT), using a commercially available fluorometer, dedicated software (Thrombinoscope®) and a calibrator. Here we explore the possibility to use platelet-rich plasma (PRP) triggered with a low amount of recombinant human tissue factor (∼0.5 pM) and also explore the function of the protein C system by adding activated protein C (APC) or soluble recombinant thrombomodulin (TM). Examples are shown: inherited antithrombin (AT) and protein C deficiencies, and antiphospholipid antibodies.