A sensitive method for detection and estimation of juvenile hormones from biological samples by glass capillary combined gas chromatography-selected ion monitoring mass spectrometry

• Published in: Analytical biochemistry Vol. 101; no. 2; pp. 356 - 363
• Main Authors: Rembold, Heinz, Hagenguth, Hartwig, Rascher, Jakob
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.01.1980
• Subjects: Animals; Chromatography, High Pressure Liquid; Chromatography, Thin Layer; Gas Chromatography-Mass Spectrometry - methods; Juvenile Hormones - analysis; Mass Spectrometry - methods; Microchemistry
• ISSN: 0003-2697; 1096-0309
• DOI: 10.1016/0003-2697(80)90200-6

Addition of n-1H,1H,2H,2H-perfluorohexanol (NFH-OH) to the epoxide ring of juvenile hormone (JH)-I, -II, -III, and ethyl-10,11-epoxyfarnesoate (JH-III-Et) yields a stable 10-hydroxy-11-NFH derivative, the mass spectrum of which contains an NFH fragment as base peak. Combination of capillary column gas chromatography-selected ion monitoring mass spectrometry makes it possible to detect and to quantify all the naturally occurring juvenile hormones from biological probes in the femtomole range. Besides high selectivity and sensitivity the method as described works faster than other procedures as extensive purification of the samples is unnecessary. By use of JH-III-Et as internal standard, interference with hormone signals in the selected ion current profile can be avoided and all the homologous juvenile hormones measured simultaneously.