Assessment of ecotoxicity effects of aspirin on non-target organism (Daphnia magna) via analysis of the responses of oxidative stress, DNA methylation-related genes expressions and life traits changes

• Published in: Ecotoxicology (London) Vol. 32; no. 2; pp. 137 - 149
• Main Authors: Cuiping, He, Na, Zhao, Limei, Hu, Tang, Tianli, Yang, Yufeng, Xiangping, Nie
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.03.2023; Springer; Springer Nature B.V
• Subjects: Acetylsalicylic acid; Animals; Anti-inflammatory agents; Antioxidants; Antioxidants - metabolism; Aquatic environment; Aquatic invertebrates; Aquatic organisms; Aspirin; Aspirin - metabolism; Aspirin - pharmacology; Biological stress; Body length; Chronic exposure; Chronic toxicity; Daphnia; Daphnia magna; Deoxyribonucleic acid; DNA; DNA Methylation; DNMT1 protein; Earth and Environmental Science; Ecology; Ecotoxicology; Environment; Environmental Management; Enzymatic activity; Enzyme activity; Freshwater crustaceans; Gene expression; Genes; Genetic research; Genetic transcription; Inflammation; Kelch-Like ECH-Associated Protein 1 - genetics; Kelch-Like ECH-Associated Protein 1 - metabolism; Methylation; Methyltransferases; NF-E2-Related Factor 2 - genetics; NF-E2-Related Factor 2 - metabolism; NF-E2-Related Factor 2 - pharmacology; Nonsteroidal anti-inflammatory drugs; Nontarget organisms; Oxidative Stress; Reproduction; Salicylates; Signal transduction; Tet gene; Toxicity; Toxicity testing; Water Pollutants, Chemical - toxicity; Antioxidant system; DNA methylation; Oxidative stress; Aspirin; Daphnia magna
• ISSN: 0963-9292; 1573-3017
• DOI: 10.1007/s10646-023-02624-z

Aspirin (acetylsalicylic acid, ASA), a widely used non-steroidal anti-inflammatory drug, was frequently detected in aquatic environments around the world. However, information on the potential toxic effects of aspirin on non-target aquatic invertebrates is limited. In the present study, we investigated the effects of ASA on the transcriptional expressions of antioxidant genes ( Nrf2 , Keap1 , HO-1 , GCLC , GPx , TRX , TrxR and Prx1 ) and DNA methylation genes ( DNMT1 , DNMT3 and TET2 ) in Daphnia magna ( D. magna )for 24, 48 and 96 h and the changes of antioxidant enzymatic activity and GSH, MDA content for 48 h. The effects of ASA on the life traits of D. magna were also addressed via a 21-days chronic toxicity test. Results showed that the expressions of Nrf2 and its target genes ( HO-1 , GPx and TrxR, GCLC , TRX and Prx1 ) were induced to different degrees at 48 h and/or 96 h. The activity of antioxidant enzymes (SOD, CAT, GST and GPx) and MDA content increased but GSH content decreased, indicating that ASA caused oxidative stress in D. magna . ASA also changed the expression of DNA methylation genes, such as DNMT and TET2 , in D. magna . We speculated that ASA may affect the antioxidant system responses through regulation of Nrf2/Keap1 signaling pathway, and/or through indirectly influencing DNA methylation levels by DNMT and TET gene expression, but the detailed mechanism needs further investigations. Chronic exposure to ASA for 21 days caused inhibitions on the growth, reproduction and behavior of D. magna (e.g., delaying days to the first brood and shortening the body length). In summary, ASA significantly affected the antioxidant responses of D. magna , and negatively disturbed its life traits in growth, development and reproduction.