The Influence of Proinflammatory Factors on the Neuroprotective Efficiency of Multipotent Mesenchymal Stromal Cells in Traumatic Brain Injury

• Published in: Bulletin of experimental biology and medicine Vol. 163; no. 4; pp. 528 - 534
• Main Authors: Danilina, T. I., Silachev, D. N., Pevzner, I. B., Gulyaev, M. V., Pirogov, Yu. A., Zorova, L. D., Plotnikov, E. Yu, Sukhikh, G. T., Zorov, D. B.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.08.2017; Springer
• Subjects: Animals; Biomedical and Life Sciences; Biomedicine; Brain damage; Brain injuries; Brain Injuries - metabolism; Brain Injuries - pathology; Cell Biology; Cells, Cultured; Cytokines; Cytokines - metabolism; Inflammation; Inflammation - metabolism; Interleukin-1alpha - metabolism; Interleukin-6 - metabolism; Internal Medicine; Laboratory Medicine; Magnetic Resonance Imaging; Male; Matrix Metalloproteinase 2 - metabolism; Matrix Metalloproteinase 9 - metabolism; Mesenchymal Stromal Cells - metabolism; Pathology; Rats; Stem cells; Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine); Tumor Necrosis Factor-alpha - metabolism; cytokines; inflammation; mesenchymal stem cells; neuroprotection
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-017-3844-5

We studied the neuroprotective potential of multipotent mesenchymal stromal cells in traumatic brain injury and the effect of inflammatory preconditioning on neuroprotective properties of stem cells under in vitro conditions. To this end, the effects of cell incubation with LPS or their co-culturing with leukocytes on production of cytokines IL-1α, IL-6, TNFα, and MMP-2 and MMP-9 by these cells were evaluated. Culturing under conditions simulating inflammation increased the production of all these factors by multipotent mesenchymal stromal cells. However, acquisition of the inflammatory phenotype by stromal cells did not reduce their therapeutic effectiveness in traumatic brain injury. Moreover, in some variants of inflammatory preconditioning, multipotent mesenchymal stromal cells exhibited more pronounced neuroprotective properties reducing the volume of brain lesion and promoting recovery of neurological functions after traumatic brain injury.