Growth hormone-releasing peptide-biotin conjugate stimulates myocytes differentiation through insulin-like growth factor-1 and collagen type I

• Published in: BMB reports Vol. 48; no. 9; pp. 501 - 506
• Main Authors: Lim, Chae Jin, Jeon, Jung Eun, Jeong, Se Kyoo, Yoon, Seok Jeong, Kwon, Seon Deok, Lim, Jina, Park, Keedon, Kim, Dae Yong, Ahn, Jeong Keun, Kim, Bong-Woo
• Format: Journal Article
• Language: English
• Published: Korea (South) Korean Society for Biochemistry and Molecular Biology 01.09.2015; 생화학분자생물학회
• Subjects: Biotin - analogs & derivatives; Biotin - chemical synthesis; Biotin - pharmacology; Cell Differentiation - drug effects; Cells, Cultured; Collagen - metabolism; Collagen Type I - metabolism; Humans; Insulin-Like Growth Factor I - metabolism; Muscle Development - drug effects; Myoblasts - cytology; Myoblasts - drug effects; Myoblasts - metabolism; Oligopeptides - chemistry; Oligopeptides - pharmacology; 화학
• ISSN: 1976-6696; 1976-670X
• DOI: 10.5483/BMBRep.2015.48.9.258

Based on the potential beneficial effects of growth hormone releasing peptide (GHRP)-6 on muscle functions, a newly synthesized GHRP-6-biotin conjugate was tested on cultured myoblast cells. Increased expression of myogenic marker proteins was observed in GHRP-6-biotin conjugate-treated cells. Additionally, increased expression levels of insulin-like growth factor-1 and collagen type I were observed. Furthermore, GHRP-6-biotin conjugate-treated cells showed increased metabolic activity, as indicated by increased concentrations of energy metabolites, such as ATP and lactate, and increased enzymatic activity of lactate dehydrogenase and creatine kinase. Finally, binding protein analysis suggested few candidate proteins, including desmin, actin, and zinc finger protein 691 as potential targets for GHRP6-biotin conjugate action. These results suggest that the newly synthesized GHRP-6-biotin conjugate has myogenic stimulating activity through, at least in part, by stimulating collagen type I synthesis and several key proteins. Practical applications of the GHRP-6-biotin conjugate could include improving muscle condition.