Effects of Selenium-Chitosan on Blood Selenium Concentration, Antioxidation Status, and Cellular and Humoral Immunity in Mice

• Published in: Biological trace element research Vol. 165; no. 2; pp. 145 - 152
• Main Authors: Qin, Shunyi, Huang, Baoxia, Ma, Jifei, Wang, Xin, Zhang, Jianbing, Li, Liuan, Chen, Fu
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.06.2015; Springer Nature B.V
• Subjects: Animals; Antioxidants; Antioxidants - metabolism; Biochemistry; Biological activity; Biomedical and Life Sciences; Biotechnology; Cell Proliferation; Chitosan - chemistry; Erythrocytes - cytology; Female; Glutathione Peroxidase - metabolism; Hemolysis; Immunity, Cellular - drug effects; Immunity, Humoral - drug effects; Immunology; Interferon-gamma - blood; Interferon-gamma - metabolism; Interleukin-2 - blood; Interleukin-2 - metabolism; Life Sciences; Male; Mice; Nutrition; Oncology; Random Allocation; Rodents; Selenium; Selenium - blood; Selenium - chemistry; Selenium Compounds - chemistry; Sheep; Sodium Selenite - chemistry; Spleen - cytology; Superoxide Dismutase; Mice; Selenium-chitosan; Immunity; Antioxidation status; Selenium concentration
• ISSN: 0163-4984; 1559-0720
• DOI: 10.1007/s12011-015-0243-5

One hundred and eighty Kunming mice were allotted to three groups in a randomized complete block design, including two treatments and one control. Mice in group 1 were fed a basal diet as control, while mice in groups 2 and 3 were fed the basal diet supplemented with 0.2 mg/kg selenium as sodium selenite (SS) or selenium-chitosan (SC), respectively. On day 28 of the experiment, blood selenium concentration, glutathione peroxidase (GPx) activity, plasma superoxide dismutase (SOD) activity, malondialdehyde (MDA) content, and Con A-induced splenocyte proliferation were determined, and plasma interleukin-2 (IL-2) and interferon-γ (IFN-γ) concentrations, splenic plaque-forming cell (PFC) responses, serum hemolysis level (HC 50 ), and delayed-type hypersensitivity (DTH) responses were determined on day 15 of the experiment. The results showed that blood selenium concentration, GPx activity, splenic PFC response, and plasma IL-2 and IFN-γ concentrations in SC group were higher than those in the control and SS groups ( P  < 0.01 or P  < 0.05), respectively. Plasma SOD activity, Serum hemolysis level, DTH responses, and Con A-induced splenocyte proliferation in SC group were higher than those in control ( P  < 0.01 or P  < 0.05). Plasma SOD activity, serum hemolysis level, DTH responses, and Con A-induced splenocyte proliferation in SC group were also higher than those in SS group, while there was no significant difference between SC and SS groups ( P  > 0.05). Plasma MDA content in SC group was lower than those in the control and SS groups ( P  < 0.01 or P  < 0.05). It is concluded that SC supplement can increase blood selenium concentration, antioxidation status, and cellular and humoral immunity, and SC has better biological activity than SS in mice.