Functionalized reduced graphene oxide with aptamer macroarray for cancer cell capture and fluorescence detection

• Published in: Mikrochimica acta (1966) Vol. 187; no. 7; p. 407
• Main Authors: Qian, Wenhui, Miao, Zhaoyi, Zhang, Xiao-Jing, Yang, Xiao-Tong, Tang, Ying-Ying, Tang, Yu Ying, Hu, Lin Yu, Li, Su, Zhu, Dong, Cheng, Haibo
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.07.2020; Springer; Springer Nature B.V
• Subjects: Analytical Chemistry; Aptamers, Nucleotide - chemistry; Base Sequence; Cancer; Cell adhesion; Cell adhesion & migration; Cell Line, Tumor; Cell Separation - methods; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Epithelial Cell Adhesion Molecule - chemistry; Ethylenediaminetetraacetic acid; Fluorescence; Fluorescence Resonance Energy Transfer; Graphene; Graphite; Graphite - chemistry; Human Umbilical Vein Endothelial Cells; Humans; Immobilized Nucleic Acids - chemistry; Limit of Detection; Microengineering; Molecular structure; Nanochemistry; Nanotechnology; Neoplastic Cells, Circulating; Original Paper; Pyrenes - chemistry; Target recognition; Aptamer macroarray; Reduced graphene oxide film; Sensitively detect cancer cells; Specifically capture cancer cells
• ISSN: 0026-3672; 1436-5073; 1436-5073
• DOI: 10.1007/s00604-020-04402-8

An integrated aptamer macroarray functionalized with reduced graphene oxide (rGO) to specifically capture and sensitively detect cancer cells is reported. The capture for cancer cells is based on effective recognition of the modified rGO surface through the aptamer against epithelial cell adhesion molecule (EpCAM). The rough structure of rGO enhances morphologic interactions between rGO film interface and the cancer cells, while super-hydrophilicity of modified rGO hinders nonspecific cell capture. The synergistic interactions offer the aptamer macroarray high efficiency of cancer cell capture. By means of a turn-on fluorescence strategy based on the conformation change of the aptamer induced by the target recognition, the enriched cancer cells can be directly read out at excitation/emission wavelengths of 550/680 nm without washing, separation, and dying steps. The working range is 1 × 10 2 to 2 × 10 4 cells per mL with a detection limit of 22 cells per mL. The results indicate that the aptamer macroarray has a considerable foreground for early diagnosis, therapy, and monitoring of cancer. Graphical abstract