Molecular cloning of a cDNA encoding mouse A15, a member of the transmembrane 4 superfamily, and its preferential expression in brain neurons

A15, a member of the transmembrane 4 superfamily (TM4SF), was isolated by differential screening of the cDNAs that are preferentially expressed on immature T cells. As a first step in the study of the biological function of the A15 molecule, we isolated cDNAs encoding the entire coding region of mou...

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Published inNeuroscience research Vol. 35; no. 4; pp. 281 - 290
Main Authors Hosokawa, Yoshitaka, Ueyama, Eiko, Morikawa, Yoshihiro, Maeda, Yumiko, Seto, Masao, Senba, Emiko
Format Journal Article
LanguageEnglish
Published Ireland Elsevier Ireland Ltd 30.12.1999
Subjects
A15
Amino Acid Sequence
Animals
Base Sequence
Brain Chemistry - genetics
CCG-B7
Cloning, Molecular
DNA, Complementary - genetics
In situ hybridization
Ion channel
Male
Membrane Proteins - genetics
Mice
Mice, Inbred C57BL
Molecular Sequence Data
Nerve Tissue Proteins
Neurons - chemistry
RNA, Messenger - analysis
Tetraspanin
TM4SF
Ion channel
In situ hybridization
A15
TM4SF
CCG-B7
Tetraspanin
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Summary:A15, a member of the transmembrane 4 superfamily (TM4SF), was isolated by differential screening of the cDNAs that are preferentially expressed on immature T cells. As a first step in the study of the biological function of the A15 molecule, we isolated cDNAs encoding the entire coding region of mouse A15. Nucleotide sequence analysis of the cDNAs revealed that mouse A15 shares 97% amino acid sequence identity with its human counterpart. The mouse A15 protein product has not yet been characterized, but is predicted to be 244 amino acids with four hydrophobic domains. Northern blot analysis of the RNA samples from various mouse tissues disclosed that the A15 transcripts are expressed most strongly in the brains, and are detectable in the colon, muscle, heart, kidney, and spleen. In situ hybridization of the mouse brain with ribo-probe established that the A15 transcripts are expressed primarily in neurons of the frontal cortex, olfactory bulb, dentate gyrus, caudoputamen, and CA3 region of the hypothalamus as well as in Purkinje cells in the cerebellar cortex, which strongly suggests that A15 may have a special function in the fundamental neuronal functioning of the higher nervous system.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0168-0102
1872-8111
DOI:10.1016/S0168-0102(99)00093-0