Quantitative determination of haloperidol in tablets by high performance thin-layer chromatography

• Published in: Journal of separation science Vol. 30; no. 5; pp. 772 - 777
• Main Authors: Mennickent, Sigrid, Pino, Loreto, Vega, Mario, Godoy, Carmen Gloria, de Diego, Marta
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01.03.2007; WILEY‐VCH Verlag; Wiley
• Subjects: Analysis; Biological and medical sciences; Chromatography, High Pressure Liquid; Chromatography, Thin Layer - methods; Drugs; General pharmacology; Haloperidol; Haloperidol - analysis; Haloperidol - chemistry; HPTLC; Medical sciences; Molecular Structure; Pharmaceutical preparations; Pharmacology. Drug treatments; Quantitative analysis; Tablets - chemistry; Validation; Drugs; Pharmaceutical preparations; Chemical analysis; Neuroleptic; Psychotropic; Dopamine receptor; Dopamine antagonist; Butyrophenone derivatives; Haloperidol; Ketone; Thin layer chromatography; D2 Dopamine receptor; Ultraviolet detector; Dosage form; Tablet; Densitometry; Quantitative analysis; HPTLC
• ISSN: 1615-9306; 1615-9314
• DOI: 10.1002/jssc.200600408

A densitometric high performance thin‐layer chromatography (HPTLC) method was developed and validated for the quantitative analysis of haloperidol in tablets. Chromatographic separation was achieved on precoated silica gel F 254 HPTLC plates using a mixture of acetone/chloroform/n‐butanol/acetic acid glacial/water (5:10:10:2.5:2.5 v/v/v/v/v) as the mobile phase. Quantitative analysis was carried out at a wavelength of 254 nm. The method was linear in the 10–100 ng/μL range, with a determination coefficient of 0.999. The coefficients of variation for precision were not higher than 2.35%. The detection limit was 0.89 ng/μL, and the quantification limit was 2.71 ng/μL. The accuracy ranged from 97.76 to 100.33%, with a CV not higher than 4.50%. This method was successfully applied to quantify haloperidol in real pharmaceutical samples, including the comparison with HPLC measurements. The method was fast, specific, with a good precision and accuracy for the quantitative determination of haloperidol in tablets.