Membrane-SPINE: An improved method to identify protein-protein interaction partners of membrane proteins in vivo
Membrane proteins are crucial for many essential cellular processes. As membrane proteins function in complexes, methods to detect and to characterize membrane protein–protein interactions are undoubtedly required. Therefore, we developed the “Membrane‐Strep‐tagged protein interaction experiment” (M...
Saved in:
Published in | Proteomics (Weinheim) Vol. 11; no. 10; pp. 2124 - 2128 |
---|---|
Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Weinheim
WILEY-VCH Verlag
01.05.2011
WILEY‐VCH Verlag Wiley Subscription Services, Inc |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Membrane proteins are crucial for many essential cellular processes. As membrane proteins function in complexes, methods to detect and to characterize membrane protein–protein interactions are undoubtedly required. Therefore, we developed the “Membrane‐Strep‐tagged protein interaction experiment” (Membrane‐SPINE) that combines the specific purification of a Strep‐tagged membrane protein with the reversible fixation of protein complexes by formaldehyde cross‐linking. In combination with MS analysis, we suggest Membrane‐SPINE as a powerful tool to identify unknown interaction partners of membrane proteins in vivo. |
---|---|
Bibliography: | Deutsche Forschungsgemeinschaft, GRAKO 1121 ark:/67375/WNG-QQFMGFM8-Q istex:364D199B1F0A66BF75F8DBFAF2EC7F974B66371B ArticleID:PMIC201000558 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1 |
ISSN: | 1615-9853 1615-9861 1615-9861 |
DOI: | 10.1002/pmic.201000558 |