Cxcl16 Interact With SARS-CoV N Protein In and Out Cell

• Published in: Virologica Sinica Vol. 25; no. 5; pp. 369 - 374
• Main Authors: Zhang, Yuan-peng, Zhang, Rong-wu, Chang, Wei-shan, Wang, Yan-yan
• Format: Journal Article
• Language: English
• Published: Heidelberg SP Wuhan Institute of Virology, CAS 01.10.2010; KeAi Publishing Communications Ltd; College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271018, China%Ling County Animal Husboundry Bureall,Dezhou 253500, China
• Subjects: Biochemistry; Biomedical and Life Sciences; Biomedicine; Calcium phosphates; Cell Line; Cell membranes; Chemokine CXCL16; Chemokines, CXC - genetics; Chemokines, CXC - metabolism; Cloning, Molecular; Confocal microscopy; CoV; CXCL16 protein; Cytoplasm; Cytoplasm - chemistry; Expression vectors; Fusion protein; Gene Expression; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; Humans; Localization; Medical Microbiology; Microbial Genetics and Genomics; Microbiology; Microscopy, Confocal; N protein; Nucleocapsid Proteins - genetics; Nucleocapsid Proteins - metabolism; N蛋白; Oncology; Plasmids; Protein Binding; Proteins; Receptors, Scavenger - genetics; Receptors, Scavenger - metabolism; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; SARS; Virology; 共聚焦激光扫描显微镜; 宿主细胞; 真核表达载体; 荧光显微镜; 蛋白相互作用; Confocal laser scanning; SARS-CoVN; Eukaryotic expression; CXCL16
• ISSN: 1674-0769; 1995-820X
• DOI: 10.1007/s12250-010-3129-x

Our study investigated the host cell protein which can interact with SARS-CoV N protein, and explored the functional connections. The eukaryotic expression vectors pEGFP-N1/SARS-CoVN and pdsRed2-N1/ CXCL16 were constructed and used to co-transfect HEK293FT cells by the calcium phosphate method. The HIS-tagged fusion protein SARS-CoVN-GFP was then built and purified for the binding assay in vitro. The co-localization of SARS-CoVN and CXCL16 in the cytoplasm of HEK293FT cells was also shown using confocal laser scanning microscopy. It is suggested that their interaction might be through direct combination Under a fluorescence microscope, it was observed that the purified fusion protein SARS-CoVN-GFP was attached to the cell membrane of CXCL16-transfected cells, indicating that SARS-CoVN and CXCL16 can be mutually combined.