Two early genes of bacteriophage T5 encode proteins containing an NTP-binding sequence motif and probably involved in DNA replication, recombination and repair

• Published in: FEBS letters Vol. 252; no. 1; pp. 47 - 52
• Main Authors: Blinov, V.M., Koonin, E.V., Gorbalenya, A.E., Kaliman, A.V., Kryukov, V.M.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 1989; Elsevier
• Subjects: Amino Acid Sequence; Bacteriophage T5, Bacteriophage T4; Biological and medical sciences; BVDV, bovine viral diarrhea virus; DNA Helicases - metabolism; DNA recombination; DNA Repair; DNA Replication; Exonuclease; Fundamental and applied biological sciences. Psychology; gp, gene product; Helicase; K2, yeast mitochondrial plasmid pGK12; M.l., Micrococcus luteus; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; NTP-binding sequence motif; Nucleoside-Triphosphatase; Phosphoric Monoester Hydrolases - metabolism; Recombination, Genetic; Replication; S.c., Saccharomyces cerevisiae; T-Phages - genetics; TEV, tobacco etch virus; Viral Proteins - genetics; VV, vaccinia virus; VZV, varicella-zoster virus; WNV, West Nile encephalitis virus; VZV, varicella-zoster virus; Helicase; DNA replication; gp, gene product; BVDV, bovine viral diarrhea virus; S.c., Saccharomyces cerevisiae; WNV, West Nile encephalitis virus; TEV, tobacco etch virus; DNA recombination; DNA repair; M.l., Micrococcus luteus; Exonuclease; Bacteriophage T5, Bacteriophage T4; NTP-binding sequence motif; K2, yeast mitochondrial plasmid pGK12; VV, vaccinia virus; Gene product; Aminoacid sequence
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(89)80887-7

It is demonstrated, by computer-assisted analysis, that T5 bacteriophage early genes D10 and D13 encode proteins containing the purine NTP-binding sequence motif. The D10 gene product is shown to be a member of a recently characterized superfamily of (putative) DNA and RNA helicases. The D13 gene product is related, at a statistically significant level, to the gene 46 product of bacteriophage T4 which is a component of an exonuclease involved in phage DNA replication, recombination and repair. A lower but also significant degree of sequence similarity was detected between the gene D12 product of T5 and the gene 47 product of T4, the second component of the same nuclease. It is hypothesized that both D10 and D13 gene products of T5 might be NTPases, possibly DNA-dependent, mediating NTP-consuming steps during phage DNA replication, recombination and/or repair.