Degradation of dibenzofuran via multiple dioxygenation by a newly isolated Agrobacterium sp. PH‐08

• Published in: Journal of applied microbiology Vol. 116; no. 3; pp. 542 - 553
• Main Authors: Le, T.T, Murugesan, K, Nam, I.‐H, Jeon, J.‐R, Chang, Y.‐S
• Format: Journal Article
• Language: English
• Published: Oxford Blackwell Science 01.03.2014; Blackwell; Oxford University Press
• Subjects: Agrobacterium; Agrobacterium - enzymology; Agrobacterium - genetics; Agrobacterium - isolation & purification; Agrobacterium sp. PH‐08; angular and lateral dioxygenation; Bacteriology; Benzofurans - metabolism; Biodegradation; Biodegradation, Environmental; Biological and medical sciences; biotransformation; byproducts; carbon; Catechol 2,3-Dioxygenase - genetics; Catechol 2,3-Dioxygenase - metabolism; cometabolism; dibenzofuran; Fundamental and applied biological sciences. Psychology; gas chromatography; Gene expression; mass spectrometry; Metabolites; Microbiology; pollutants; Tandem Mass Spectrometry; Thiophenes - metabolism; Degradation; Agrobacterium; angular and lateral dioxygenation; dibenzofuran; Applied microbiology; PH-08; pH; Bacteria; Rhizobiaceae; Cometabolism; Agrobacterium sp; Agrobacterium sp. PH-08; cometabolism
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1111/jam.12403

AIMS: To demonstrate the biodegradation of dibenzofuran (DF) and its structural analogs by a newly isolated Agrobacterium sp. PH‐08. METHODS AND RESULTS: To assess the biodegradation potential of newly isolated Agrobacterium sp. PH‐08, various substrates were evaluated as sole carbon sources in growth and biotransformation experiments. ESI LC‐MS/MS analysis revealed the presence of angular degrading by‐products as well as lateral dioxygenation metabolites in the upper pathway. The metabolites in the lower pathway also were detected. In addition, the cometabolically degraded daughter compounds of DF‐related compounds such as BP and dibenzothiophene (DBT) in dual substrate degradation were observed. Strain PH‐08 exhibited the evidence of meta‐cleavage pathway as confirmed by the activity and gene expression of catechol‐2,3‐dioxygenase. CONCLUSIONS: Newly isolated bacterial strain, Agrobacterium sp. PH‐08, grew well with and degraded DF via both angular and lateral dioxygenation as demonstrated by metabolites identified through ESI LC‐MS/MS and GC‐MS analyses. The other heterocyclic pollutants were also cometabolically degraded. SIGNIFICANCE AND IMPACT OF THE STUDY: Few reports have described the complete degradation of DF by a cometabolic lateral pathway. Our study demonstrates the novel results that the newly isolated strain utilized the DF as a sole carbon source and mineralized it via multiple dioxygenation.