Isolation of Antifungal Compound from Paeonia Suffruticosa and Its Antifungal Mechanism

• Published in: Chinese journal of integrative medicine Vol. 21; no. 3; pp. 211 - 216
• Main Author: 赵勇 王宝恩 张淑文 杨淑敏 王红 任爱民 衣恩通
• Format: Journal Article
• Language: English
• Published: Heidelberg Chinese Association of Traditional and Western Medicine 01.03.2015
• Subjects: Antifungal Agents - chemistry; Antifungal Agents - isolation & purification; Antifungal Agents - pharmacology; Candida - drug effects; Candida - ultrastructure; Chromatography, High Pressure Liquid; Hydrolyzable Tannins - chemistry; Hydrolyzable Tannins - isolation & purification; Hydrolyzable Tannins - pharmacology; Mass Spectrometry; Medicine; Medicine & Public Health; Microbial Sensitivity Tests; Original Article; Paeonia - chemistry; Plant Bark - chemistry; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Plant Roots - chemistry; Proton Magnetic Resonance Spectroscopy; 化合物; 抗真菌活性; 机制; 牡丹; 生物测定方法; 萃取分离; 透射电子显微镜; 高效液相色谱; 1,2,3,4,6-penta-O-galloyl-β-D-glucose; antifungal activity
• ISSN: 1672-0415; 1993-0402; 1993-0402
• DOI: 10.1007/s11655-014-1805-7

Objective：To isolate antifungal compound from Paeonia suffruticosa,and to find the antifungal mechanisms by observing the ultrastructural modifications of yeasts in growth phase produced by 1,2,3,4,6-pentaO-galloyl-beta-D-glucose（PGG）.Methods：Peony（Paeonia suffruticosa） root bark（PRB） was separated by solvent extraction and purified by high performance liquid chromatography（HPLC） method using analytical and preparative reversed phase C18 column on the basis of bio-assay method.In order to investigate the antifungal mechanism of PGG,Yeasts were submitted to different concentrations[3×minimum inhibition concentration（MIC）,0.3×MIC]for 1 h under constant stirring at 30 ℃,and transmission electron microscopy was performed.Results：Based on the antifungal activity of PRB on Candida glabrata CBS138,the antifungal compound were isolated in ethyl acetate layer of PRB and identified as PGG by mass spectrometry,1H nuclear magnetic resonance（NMR） analyses,with molecular weight of 940 and molecular formular as C41H32O26.Transmission electron microscopy showed that PGG degraded the cell wall envelope.Conclusion：The results suggest that PGG may be responsible for the antifungal activity of PRB by disrupting the structure of cell wall directly.