Characterization of vitamin K from bovine liver

Concentrated fractions of vitamin K from bovine liver were purified by thin-layer chromatography and fractions were analyzed by UV spectroscopy and mass spectrometry. The chromatographic behavior of the purified vitamins was compared with that of known compounds on thin layers of silica gel, either...

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Bibliographic Details
Published inJournal of lipid research Vol. 9; no. 2; pp. 176 - 179
Main Authors Matschiner, J T, Amelotti, J M
Format Journal Article
LanguageEnglish
Published United States Elsevier 01.03.1968
Subjects
Animals
bovine liver
Cattle
Chromatography, Thin Layer
lipophilic naphthoquinones
Liver - analysis
mass spectrometry
menaquinones-10, 11, 12
Silicon Dioxide
Spectrophotometry
Spectrum Analysis
thin-layer chromatography
Ultraviolet Rays
vitamin K
Vitamin K - analysis
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Summary:Concentrated fractions of vitamin K from bovine liver were purified by thin-layer chromatography and fractions were analyzed by UV spectroscopy and mass spectrometry. The chromatographic behavior of the purified vitamins was compared with that of known compounds on thin layers of silica gel, either untreated or impregnated with silver nitrate or paraffin. The principal forms of vitamin K recovered from bovine liver were highly lipophilic. Two fractions were obtained which collectively gave identifiable mass spectra of menaquinone-10, menaquinone-11, and menaquinone-12.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0022-2275
DOI:10.1016/S0022-2275(20)43116-5