Amperometric fructose sensor based on ferrocyanide modified screen-printed carbon electrode

• Published in: Talanta (Oxford) Vol. 88; pp. 432 - 438
• Main Authors: Biscay, Julien, Costa Rama, Estefanía, González García, María Begoña, Julio Reviejo, A., Pingarrón Carrazón, José Manuel, García, Agustín Costa
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 15.01.2012; Elsevier
• Subjects: Analytical chemistry; Biosensing Techniques; Calibration; Carbohydrate Dehydrogenases; Carbon - chemistry; Chemistry; Electrochemical methods; Electrochemistry; Electrodes; Enzymes, Immobilized; Exact sciences and technology; Ferrocyanide; Ferrocyanides - chemistry; Fructose - analysis; Fructose dehydrogenase; Fructose sensor; Fruit - chemistry; General, instrumentation; Honey - analysis; Hydrogen-Ion Concentration; Kinetics; Limit of Detection; Reproducibility of Results; Screen-printed carbon electrode; Signal-To-Noise Ratio; Wine - analysis; Fructose dehydrogenase; Ferrocyanide; Fructose sensor; Screen-printed carbon electrode; Wine; Ink; Immobilization; Accuracy; Detection limit; Amperometry; Pretreatment; Stability; Enzyme; Sample; Carbon electrode; Chemical sensor; Carbon; Fruit juice; Fructose; Electrochemical detector; Honey; Apple; Sensitivity; Reproducibility; Hexacyanoferrates II; Room temperature
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2011.11.013

► Generation in situ of FDH mediator by oxidation of ferrocyanide of the electrode. ► Sensor pre-treatment allows a better sensitivity and reproducibility. ► Screen-printed fructose sensor with the longest stability, minimum of 2 months. ► Sensor is specific to fructose without any interferences caused by other sugars. The first fructose sensor using a commercial screen-printed ferrocyanide/carbon electrodes (SPFCE) is reported here. The ferrocyanide is included in the carbon ink of the commercial screen-printed carbon electrode. The immobilization of enzyme d-fructose dehydrogenase (FDH) was carried out in an easy way. An aliquot of 10μL FDH was deposited on the electrode surface and left there until dried (approximately 1h) at room temperature. The sensor, so constructed, shows a good sensitivity to fructose (1.25μA/mM) with a slope deviation of ±0.02μA/mM and a linear range comprised between 0.1 and 1mM of fructose, with a limit of detection of 0.05mM. These sensors show good intersensors reproducibility after a previous pretreatment and a high stability. Fructose was determined in real samples as honey, Cola, fruit juices (orange, tomato, apple and pineapple), red wine, red and white grapes, musts and liquor of peach with a good accuracy.