Chemical composition and cytotoxic activity of Garcinia atroviridis Griff. ex T. Anders. essential oils in combination with tamoxifen

• Published in: Natural product research Vol. 32; no. 7; pp. 854 - 858
• Main Authors: Tan, Wen-Nee, Lim, Jia-Qin, Afiqah, Fatin, Nik Mohamed Kamal, Nik Nur Syazni, Abdul Aziz, Fatin Athirah, Tong, Woei-Yenn, Leong, Chean-Ring, Lim, Jun-Wei
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 03.04.2018; Taylor & Francis Ltd
• Subjects: Apoptosis; Bark; breast cancer; Caryophyllene; Cell death; Chemical composition; Cytotoxicity; Drugs; Essential oils; Farnesene; Garcinia; Garcinia atroviridis; Leaves; Palmitic acid; Palmitoleic acid; Tamoxifen; Garcinia atroviridis; breast cancer; essential oils; cytotoxicity; tamoxifen
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2017.1361951

Garcinia atroviridis Griff. ex T. Anders. is used as a medication agent in folkloric medicine. The present study was to examine the chemical composition of the stem bark and leaf of G. atroviridis as well as their cytotoxic effects against MCF-7 cells. The constituents obtained by hydrodistillation were identified using GC-MS. The stem bark oil (EO-SB) composed mainly the palmitoleic acid (51.9%) and palmitic acid (21.9%), while the leaf oil (EO-L) was dominated by (E)-β-farnesene (58.5%) and β-caryophyllene (16.9%). Treatment of MCF-7 cells using EO-L (100 μg/mL) caused more than 50% cell death while EO-SB did not induce cytotoxic effect. EO-L has stimulated the growth of BEAS-2B normal cells, but not in MCF-7 cancerous cells. The IC 50 of EO-L in MCF-7 and BEAS-2B cells were 71 and 95 μg/mL, respectively. A combination treatment of EO-L and tamoxifen induced more cell death than the treatment with drug alone at lower doses.