Antibody Binding to SARS-CoV-2 S Glycoprotein Correlates with but Does Not Predict Neutralization

• Published in: Viruses Vol. 12; no. 11; p. 1214
• Main Authors: Ding, Shilei, Laumaea, Annemarie, Benlarbi, Mehdi, Beaudoin-Bussières, Guillaume, Gasser, Romain, Medjahed, Halima, Pancera, Marie, Stamatatos, Leonidas, McGuire, Andrew T, Bazin, Renée, Finzi, Andrés
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI 26.10.2020; MDPI AG
• Subjects: Animals; Antibodies, Immobilized - immunology; Antibodies, Monoclonal - immunology; Antibodies, Neutralizing - immunology; Antibodies, Viral - immunology; Betacoronavirus - immunology; Cell Line; Communication; Convalescence; convalescent plasma; Coronavirus Infections - immunology; COVID-19; ELISA; HEK293 Cells; Humans; neutralization; Neutralization Tests; Pandemics; Pneumonia, Viral - immunology; SARS-COV-2; Spike Glycoprotein, Coronavirus - immunology; Time Factors; virus capture assay; COVID-19; SARS-COV-2; neutralization; virus capture assay; ELISA; convalescent plasma
• ISSN: 1999-4915; 1999-4915
• DOI: 10.3390/v12111214

Convalescent plasma from SARS-CoV-2 infected individuals and monoclonal antibodies were shown to potently neutralize viral and pseudoviral particles carrying the S glycoprotein. However, a non-negligent proportion of plasma samples from infected individuals, as well as S-specific monoclonal antibodies, were reported to be non-neutralizing despite efficient interaction with the S glycoprotein in different biochemical assays using soluble recombinant forms of S or when expressed at the cell surface. How neutralization relates to the binding of S glycoprotein in the context of viral particles remains to be established. Here, we developed a pseudovirus capture assay (VCA) to measure the capacity of plasma samples or antibodies immobilized on ELISA plates to bind to membrane-bound S glycoproteins from SARS-CoV-2 expressed at the surface of lentiviral particles. By performing VCA, ELISA, and neutralization assays, we observed a strong correlation between these parameters. However, while we found that plasma samples unable to capture viral particles did not neutralize, capture did not guarantee neutralization, indicating that the capacity of antibodies to bind to the S glycoprotein at the surface of pseudoviral particles is required but not sufficient to mediate neutralization. Altogether, our results highlight the importance of better understanding the inactivation of S by plasma and neutralizing antibodies.