Effect of castalagin against HSV-1 infection in newborn mice

• Published in: Natural product research Vol. ahead-of-print; no. ahead-of-print; pp. 1 - 6
• Main Authors: Stoyanova, Adelina, Popatanasov, Аndrey, Rashev, Viktor, Tancheva, Lyubka, Quideau, Stephane, Galabov, Angel S.
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 17.12.2023; Taylor & Francis Ltd
• Subjects: Acute toxicity; Acyclovir; Biocompatibility; castalagin; Herpes simplex virus type 1; mice; Quercus robur; Resistant mutant; Synergistic effect; Toxicity; toxicology; toxicology; castalagin; Herpes simplex virus type 1; mice
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2023.2173191

We tested in vivo anti-herpetic effect of castalagin, an ellagitannin compound, extracted from pedunculate oak (Quercus robur). Previous investigations found that castalagin possesses a strong inhibitory effect in vitro against HSV-1/2 equal to acyclovir (ACV). It is also effective against ACV-resistant mutants and shows a synergistic effect with ACV. We study castalagin's activity towards HSV-1 infection in newborn mice. Acute toxicity determination in mice showed LD 50 value of 295 mg/kg. Prolonged toxicity was also constructed. Castalagin manifested a marked activity against HSV-1 (LD 90 /0.02 ml) administered in 7-day course at 0.02 ml s.c. doses of 7.5 or 10 mg/kg (PI 57-58%). ACV course demonstrated a marked activity at 20 mg/kg. The selectivity ratio LD 50 /ED 50 (295/7.5) could be accepted as ≥ 33.