Chymase activities and survival in endotoxin-induced human chymase transgenic mice

• Published in: International journal of medical sciences Vol. 11; no. 3; pp. 222 - 225
• Main Authors: Rafiq, Kazi, Fan, Yu-Yan, Sherajee, Shamshad J, Takahashi, Yoshimasa, Matsuura, Junji, Hase, Naoki, Mori, Hirohito, Nakano, Daisuke, Kobara, Hideki, Hitomi, Hirofumi, Masaki, Tsutomu, Urata, Hidenori, Nishiyama, Akira
• Format: Journal Article
• Language: English
• Published: Australia Ivyspring International Publisher 01.01.2014
• Subjects: Animals; Chymases - biosynthesis; Chymases - genetics; Gene Expression Regulation, Enzymologic - drug effects; Humans; Lipopolysaccharides - toxicity; Mice, Transgenic; Myocardium - enzymology; Short Research Communication; Skin - drug effects; Skin - enzymology; Survival; human chymase transgenic mice; chymase activity and lipopolysaccharide; endotoxemia
• ISSN: 1449-1907; 1449-1907
• DOI: 10.7150/ijms.7382

We examined the effects of overexpressed human chymase on survival and activity in lipopolysaccharide (LPS)-treated mice. Human chymase transgenic (Tg) and wild-type C57BL/6 (WT) mice were treated with LPS (0.03, 0.1 and 0.3 mg/day; intraperitoneal) for 2 weeks. Treatment with 0.03 mg LPS did not affect survival in either WT or Tg mice. WT mice were not affected by 0.1 mg/day of LPS, whereas 25% of Tg mice died. Survival of mice treated with 0.3 mg/day of LPS was 87.5% and 0% in WT and Tg, respectively. LPS-induced increases in chymase activity in the heart and skin were significantly greater in Tg than WT mice. These data suggest a possible contribution of human chymase activation to LPS-induced mortality.