Plant-based production of a protective vaccine antigen against the bovine parasitic nematode Ostertagia ostertagi

The development of effective recombinant vaccines against parasitic nematodes has been challenging and so far mostly unsuccessful. This has also been the case for Ostertagia ostertagi, an economically important abomasal nematode in cattle, applying recombinant versions of the protective native activ...

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Published inScientific reports Vol. 13; no. 1; p. 20488
Main Authors Zwanenburg, Laurens, Borloo, Jimmy, Decorte, Bregt, Bunte, Myrna J M, Mokhtari, Sanaz, Serna, Sonia, Reichardt, Niels-C, Seys, Leen J M, van Diepen, Angela, Schots, Arjen, Wilbers, Ruud H P, Hokke, Cornelis H, Claerebout, Edwin, Geldhof, Peter
Format Journal Article
LanguageEnglish
Published England Nature Publishing Group 22.11.2023
Nature Portfolio
Subjects
Animals
Antibodies
Antigens
Cattle
Cattle Diseases
Chromatography
Economic importance
Glycosylation
Immune response
Immunization
Infections
Nematodes
Ostertagia - genetics
Ostertagia ostertagi
Ostertagiasis - prevention & control
Ostertagiasis - veterinary
Oxidation
Parasite Egg Count
Parasites
Parasitology
Peptides
Protein folding
Protein structure
Recombinant Proteins - genetics
Recombinants
Vaccination - veterinary
Vaccines
Vaccines, Synthetic - genetics
Veterinary medicine
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Summary:The development of effective recombinant vaccines against parasitic nematodes has been challenging and so far mostly unsuccessful. This has also been the case for Ostertagia ostertagi, an economically important abomasal nematode in cattle, applying recombinant versions of the protective native activation-associated secreted proteins (ASP). To gain insight in key elements required to trigger a protective immune response, the protein structure and N-glycosylation of the native ASP and a non-protective Pichia pastoris recombinant ASP were compared. Both antigens had a highly comparable protein structure, but different N-glycan composition. After mimicking the native ASP N-glycosylation via the expression in Nicotiana benthamiana plants, immunisation of calves with these plant-produced recombinants resulted in a significant reduction of 39% in parasite egg output, comparable to the protective efficacy of the native antigen. This study provides a valuable workflow for the development of recombinant vaccines against other parasitic nematodes.
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ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-023-47480-3