Incorporated sarcolemmal fish oil fatty acids shorten pig ventricular action potentials

• Published in: Cardiovascular research Vol. 70; no. 3; pp. 509 - 520
• Main Authors: VERKERK, Arie O, VAN GINNEKEN, Antoni C. G, FIOLET, Jan W. T, ZOCK, Peter L, CORONEL, Ruben, BERECKI, Géza, DEN RUIJTER, Hester M, SCHUMACHER, Cees A, VELDKAMP, Marieke W, BAARTSCHEER, Antonius, CASINI, Simona, OPTHOF, Tobias, HOVENIER, Robert
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.06.2006
• Subjects: 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; Action Potentials - physiology; adult; Animals; Antioxidants - pharmacology; arrhythmias; Arrhythmias, Cardiac - metabolism; Biological and medical sciences; Calcium Channel Blockers - pharmacology; Calcium Channels - metabolism; Cardiology. Vascular system; chloride current; Chromans - pharmacology; Diet; dietary supplementation; exchange; Fatty Acids, Omega-3 - administration & dosage; Fish Oils; guinea-pig; Heart Ventricles; heart-failure; inhibition; ischemia; Male; Medical sciences; Membrane Potentials - physiology; myocytes; Myocytes, Cardiac - metabolism; Myocytes, Cardiac - physiology; Nifedipine - pharmacology; Patch-Clamp Techniques; Sarcolemma - metabolism; Sarcolemma - physiology; Sodium Channels - metabolism; Sodium-Calcium Exchanger - physiology; Swine; Time Factors; Repolarization; Nutrition; Action potential; Fatty acids; Fish oil; Phlebology; Pig; Electrodiagnosis; Vertebrata; Mammalia; Ion exchangers; Animal; Ion channels; Artiodactyla; Circulatory system; Membrane potential; Cardiology; Ca2+ transients; Ungulata
• ISSN: 0008-6363; 1755-3245
• DOI: 10.1016/j.cardiores.2006.02.022

Omega-3 polyunsaturated fatty acids (omega3-PUFAs) from fish oil reduce the risk of sudden death presumably by preventing life-threatening arrhythmias. Acutely administered omega3-PUFAs modulate the activity of several cardiac ion channels, but the chronic effects of a diet enriched with fish oil leading to omega3-PUFA-incorporation into the sarcolemma on membrane currents are unknown. Pigs received a diet either rich in omega3-PUFAs or in omega9-fatty acids for 8 weeks. Ventricular myocytes (VMs) were isolated and used for patch-clamp studies. omega3-VMs contained higher amounts of omega3-PUFAs and had a shorter action potential (AP) with a more negative plateau than control VM. In omega3 VMs, L-type Ca(2+) current (I(Ca,L)) and Na(+)-Ca(2+) exchange current (I(NCX)) were reduced by approximately 20% and 60%, respectively, and inward rectifier K(+) current (I(K1)) and slow delayed rectifier K(+) current (I(Ks)) were increased by approximately 50% and 70%, respectively, compared to control. Densities of rapid delayed rectifier K(+) current, Ca(2+)-activated Cl(-) current, and Na(+) current (I(Na)) were unchanged, although voltage-dependence of I(Na) inactivation was more negative in omega3 VMs. A fish oil diet increases omega3-PUFA content in the ventricular sarcolemma, decreases I(Ca,L) and I(NCX), and increases I(K1) and I(Ks), resulting in AP shortening. Incorporation of omega3-PUFAs in the sarcolemma may have consequences for arrhythmias independent of circulating omega3-PUFAs.