Effect of cultured autologous oral keratinocyte suspension in fibrin glue on oral wound healing in rabbits

• Published in: International journal of oral and maxillofacial surgery Vol. 41; no. 9; pp. 1146 - 1152
• Main Authors: Lis, G.J, Zarzecka, J, Litwin, J.A, Jasek, E, Cichocki, T, Zapała, J
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.09.2012; Elsevier
• Subjects: Animals; Biological and medical sciences; Biological Dressings; Cells, Cultured; Combined Modality Therapy; Dentistry; fibrin glue; Fibrin Tissue Adhesive - administration & dosage; keratinocyte culture; Keratinocytes - cytology; Keratinocytes - transplantation; Medical sciences; Mouth Mucosa - drug effects; Mouth Mucosa - surgery; oral mucosa; Otorhinolaryngology. Stomatology; Rabbits; Surgery; Transplantation, Autologous; wound healing; Wound Healing - drug effects; Wound Healing - physiology; keratinocyte culture; fibrin glue; wound healing; oral mucosa; Cell culture; Hemostatic; Stomatology; Mucosa; Oral administration; Rabbit; Suspension; Autologous system; Lagomorpha; Wound; Oral cavity; Fibrin; Vertebrata; Mammalia; Treatment; Healing agent; Animal; Surgery; Keratinocyte; Cicatrization
• ISSN: 0901-5027; 1399-0020
• DOI: 10.1016/j.ijom.2012.02.017

Abstract The effect of cultured autologous oral keratinocyte suspension in fibrin glue on the healing of surgically produced oral mucosal wounds was assessed in the rabbit model. Using the light microscope and a digital image analysis system, the epithelization parameters (marginal epithelization and percentage of wound re-epithelization) were measured in haematoxylin–eosin stained sections of the wound area and compared with those of wounds treated with fibrin glue alone and untreated ones. The epithelization was significantly higher in keratinocytes plus fibrin glue-treated wounds on postoperative days 3 and 7. No significant differences were observed on postoperative day 1, when the healing process had just begun, and on postoperative day 14, when re-epithelization was completed or nearly completed in all groups. The inflammatory infiltration of the wounded mucosa was weakest in keratinocyte-treated wounds and strongest in untreated wounds. In conclusion, suspension of cultured autologous oral keratinocytes in fibrin glue significantly accelerates oral wound healing in the rabbit model and could be beneficial in the treatment of oral wounds in patients.