Differential modulation of macrophage response elicited by glycoinositolphospholipids and lipophosphoglycan from Leishmania ( Viannia ) shawi

• Published in: Parasitology international Vol. 64; no. 4; pp. 32 - 35
• Main Authors: Passero, Luiz F.D, Assis, Rafael R, da Silva, Thays N.F, Nogueira, Paula M, Macedo, Diego H, Pessoa, Natália L, Campos, Marco A, Laurenti, Márcia D, Soares, Rodrigo P
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ireland Ltd 01.08.2015
• Subjects: Animals; Gastroenterology and Hepatology; Glycoconjugates - immunology; Glycoinositolphospholipids; Glycosphingolipids - chemistry; Glycosphingolipids - immunology; Host-Parasite Interactions; Infectious Disease; Interaction; Interleukin-10 - metabolism; Interleukin-12 - metabolism; Leishmania (Viannia) shawi; Leishmania - chemistry; Leishmania - immunology; Lipophosphoglycan; Macrophages, Peritoneal - immunology; Mice, Inbred BALB C; Nitric Oxide - metabolism; Phosphatidylinositols - chemistry; Phosphatidylinositols - immunology; Tumor Necrosis Factor-alpha - metabolism; Leishmania ( Viannia) shawi; Interaction; Glycoinositolphospholipids; Lipophosphoglycan; Leishmania (Viannia) shawi
• ISSN: 1383-5769; 1873-0329
• DOI: 10.1016/j.parint.2015.01.006

Abstract In this work, some aspects of the glycobiology of Leishmania shawi were examined, as it is a causative agent of cutaneous leishmaniasis in the New World. Additionally, the interaction of L. shawi 's main glycoconjugates [lipophosphoglycan (LPG) and glycoinositolphospholipids (GIPLs)] with macrophages was evaluated in vitro. L. shawi LPG was devoid of side-chains in its repeat units, whereas monosaccharide analysis showed that GIPLs were suggestive of mannose-rich (type I or hybrid). In order to evaluate the biological roles of those molecules, BALB/c resident peritoneal macrophages were incubated with these glycoconjugates for 24 h, and the levels of nitric oxide (NO), tumor necrosis factor (TNF)-α, interleukin (IL)-12p70 and IL-10, were determined. In general, the GIPLs exhibited a greater proinflammatory role than the LPGs did. However, for the first time, the GIPLs from this species were able to trigger the production of IL-10, an anti-inflammatory cytokine. In conclusion, L. shawi glycoconjugates were able to interact with the innate immune compartment. These data reinforce the role of parasite glycoconjugates during parasite and host cell interactions.