Characterization of a novel marine aerobic denitrifier Vibrio spp. AD2 for efficient nitrate reduction without nitrite accumulation

• Published in: Environmental science and pollution research international Vol. 28; no. 24; pp. 30807 - 30820
• Main Authors: Ren, Jilong, Ma, Hongjing, Liu, Ying, Ruan, Yunjie, Wei, Chenzheng, Song, Jing, Wu, Yinghai, Han, Rui
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.06.2021; Springer Nature B.V
• Subjects: Accumulation; Aerobic conditions; Aquaculture; Aquatic Pollution; Atmospheric Protection/Air Quality Control/Air Pollution; Bacteria; Carbon/nitrogen ratio; Denitrification; Earth and Environmental Science; Ecotoxicology; Environment; Environmental Chemistry; Environmental Health; Environmental science; Experiments; Genes; Nitrate reductase; Nitrate reduction; Nitrates; Nitrogen; Nitrogen balance; Nitrogen dioxide; Nitrous oxide; Organic nitrogen; Polymerase chain reaction; Reductases; Research Article; Response surface methodology; Salinity tolerance; Shaking; Vibrio; Waste Water Technology; Wastewater; Water Management; Water pollution; Water Pollution Control; spp; Nitrogen balance; Aerobic denitrification; Denitrification gene; Nitrogen removal; Response surface methodology; AD2; Vibrio spp
• ISSN: 0944-1344; 1614-7499
• DOI: 10.1007/s11356-021-12673-8

Aerobic denitrifiers have the potential to reduce nitrate in polluted water under aerobic conditions. A salt-tolerant aerobic denitrifier was newly isolated and identified as Vibrio spp . AD2 from a marine recirculating aquaculture system, in which denitrification performance was investigated via single-factor experiment, Box–Behnken experiment, and nitrogen balance analysis. Nitrate reductase genes were identified by polymerase chain reaction. Results showed that strain AD2 removed 98.9% of nitrate-nitrogen (NO 3 − -N) with an initial concentration about 100 mg·L −1 in 48 h without nitrite-nitrogen (NO 2 − -N) accumulation. Nitrogen balance indicated that approximately 17.5% of the initial NO 3 − -N was utilized for bacteria synthesis themselves, 4.02% was converted to organic nitrogen, 39.8% was converted to nitrous oxide (N 2 O), and 31.1% was converted to nitrogen (N 2 ). Response surface methodology experiment showed that the maximum removal of total nitrogen (TN) occurred under the condition of C/N ratio 11.5, shaking speed 127.9 rpm, and temperature 30.8 °C. Sequence amplification indicated that the denitrification genes, napA and nirS , were present in strain AD2. These results indicated that the strain AD2 has potential applications for removing NO 3 − -N from high-salinity (3%) wastewater.