Glutathione Fluorescence Sensing Based on a Co-Doped Carbon Dot/Manganese Dioxide Nanocoral Composite

• Published in: Materials Vol. 15; no. 23; p. 8677
• Main Authors: Le, Thi-Hoa, Lee, Hyun-Jong, Tran, Quang-Nhat
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 05.12.2022; MDPI
• Subjects: Absorption spectra; Alzheimer's disease; Carbon; Carbon dots; Cells; Composite materials; Cysteine; Energy transfer; Fluorescence; fluorescence resonance energy transfer (FRET); Glutathione; Manganese dioxide; Morphology; NPCD-MnO2 nanocoral composite; Pathogenesis; Reducing agents; Sensors; Spectrum analysis; Thiols; “turn on” fluorescence; United States > US; Japan; NPCD-MnO2 nanocoral composite; “turn on” fluorescence; fluorescence resonance energy transfer (FRET); Glutathione
• ISSN: 1996-1944; 1996-1944
• DOI: 10.3390/ma15238677

Glutathione (GSH) is an antioxidant thiol that has a vital role in the pathogenesis of various human diseases such as cardiovascular disease and cancer. Hence, it is necessary to study effective methods of GSH evaluation. In our work, an effective GSH sensor based on a nitrogen and phosphorus co-doped carbon dot (NPCD)-MnO nanocoral composite was fabricated. In addition to utilizing the strong fluorescence of the NPCDs, we utilized the reductant ability of the NPCDs themselves to form MnO and then the NPCD-MnO nanocoral composite from MnO . The characteristics of the nanocoral composite were analyzed using various electron microscopy techniques and spectroscopic techniques. The overlap between the absorption spectrum of MnO and the fluorescence emission spectrum of the NPCDs led to effective fluorescence resonance energy transfer (FRET) in the nanocoral composite, causing a decrease in the fluorescent intensity of the NPCDs. A linear recovery of the fluorescent intensity of the NPCDs was observed with the GSH level raising from 20 to 250 µM. Moreover, our GSH sensor showed high specificity and sensing potential in real samples with acceptable results.