Toxicological evaluation in silico and in vivo of secondary metabolites of Cissampelos sympodialis in Mus musculus mice following inhalation

• Published in: Natural product research Vol. 33; no. 6; pp. 789 - 795
• Main Authors: Alves, Mateus Feitosa, Ferreira, Larissa Adilis Maria Paiva, Gadelha, Francisco Allysson Assis Ferreira, Ferreira, Laércia Karla Diega Paiva, Felix, Mayara Barbalho, Scotti, Marcus Tullius, Scotti, Luciana, de Oliveira, Kardilândia Mendes, dos Santos, Sócrates Golzio, Diniz, Margareth de Fátima Formiga Melo
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 19.03.2019; Taylor & Francis Ltd
• Subjects: Alkaloids; Biocompatibility; Cissampelos; Cissampelos sympodialis; Immunomodulation; in silico; in vivo; Inflammation; infuse; Inhalation; LC-MS/MS; Leaves; Metabolism; Metabolites; Mice; Pharmacology; Respiration; Rodents; Secondary metabolites; Toxicity; toxicity; LC-MS/MS; inhalation; infuse
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2017.1410805

The ethanolic extract of the leaves of Cissampelos sympodialis showed great pharmacological potential, with inflammatory and immunomodulatory activities, however, it showed some toxicological effects. Therefore, this study aims to verify the toxicological potential of alkaloids of the genus Cissampelos through in silico methodologies, to develop a method in LC-MS/MS verifying the presence of alkaloids in the infusion and to evaluate the toxicity of the infusion of the leaves of C. sympodialis when inhaled by Swiss mice. Results in silico showed that alkaloid 93 presented high toxicological potential along with the products of its metabolism. LC-MS/MS results showed that the infusion of the leaves of this plant contained the alkaloids warifteine and methylwarifteine. Finally, the in vivo toxicological analysis of the C. sympodialis infusion showed results, both in biochemistry, organ weights and histological analysis, that the infusion of C. sympodialis leaves presents a low toxicity.