Histological effects on the kidney, spleen, and liver of Nile tilapia Oreochromis niloticus fed different concentrations of probiotic Lactobacillus plantarum

• Published in: Tropical animal health and production Vol. 52; no. 1; pp. 167 - 176
• Main Authors: Ruiz, Maria Luiza, Owatari, Marco Shizuo, Yamashita, Marcela Maya, Ferrarezi, José Victor Saffadi, Garcia, Patricia, Cardoso, Lucas, Martins, Maurício Laterça, Mouriño, José Luiz Pedreira
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.01.2020; Springer Nature B.V
• Subjects: Animal Feed - analysis; Animals; Biomedical and Life Sciences; Cichlids - physiology; Congestion; Diet; Diet - veterinary; Dose-Response Relationship, Drug; Feed additives; Fish; Goblet cells; Growth rate; Immune system; Kidney - drug effects; Kidneys; Lactobacillus plantarum; Lactobacillus plantarum - chemistry; Lesions; Leukocytes; Life Sciences; Liver; Liver - drug effects; Oreochromis niloticus; Organs; Parameters; Ponds; Probiotics; Probiotics - administration & dosage; Reduction; Regular Articles; Spleen; Spleen - drug effects; Tilapia; Veterinary Medicine/Veterinary Science; Zoology; Fish health; Sustainable fish farming; Aquaculture; Oreochromis niloticus
• ISSN: 0049-4747; 1573-7438
• DOI: 10.1007/s11250-019-02001-1

The aims of this study were to evaluate the inclusion of different concentrations of Lactobacillus plantarum in Nile tilapia diet and to verify histological effects on tissues of the animal organs, as well as to verify its effects on growth parameters and possible increase in the immune system. A total of 240 juveniles were distributed in 16 tanks arranged in a recirculation system. One control group and three treated groups (10 4 , 10 6 , and 10 8 colony-forming unit (CFU) g −1 L. plantarum kg feed −1 ) were established in quadruplicate. After 35 days of supplementation, it was not possible to observe differences in growth rates and hematological parameters. However, in the kidney, there was a reduction in the presence of PAS-positive granular leukocytes (PAS-GL) between the collections (15 and 35 days). The liver had lower number of lesions related to loss of cordonal aspects of fish fed 10 8  CFU g −1 on day 15. Fish fed 10 4 and 10 8  CFU g −1 showed lower degree of congestion at day 35. The probiotic also provided a reduction in the number of melanomacrophage centers in the splenic tissue and an increase in the amount of goblet cells in the gut. The concentration 10 8  CFU g −1 of probiotic in diets increased the number of goblet cells, improved cordonal aspects, and reduced hepatic congestion, and indicated a possible improvement in the immunophysiological conditions of the fish.