Biological synthesis of gold and silver chloride nanoparticles by Glycyrrhiza uralensis and in vitro applications

• Published in: Artificial cells, nanomedicine, and biotechnology Vol. 46; no. 2; pp. 303 - 312
• Main Authors: Huo, Yue, Singh, Priyanka, Kim, Yeon Ju, Soshnikova, Veronika, Kang, Jongpyo, Markus, Josua, Ahn, Sungeun, Castro-Aceituno, Verónica, Mathiyalagan, Ramya, Chokkalingam, Mohan, Bae, Kwi-Sik, Yang, Deok Chun
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 01.03.2018; Taylor & Francis Ltd
• Subjects: Anticancer properties; antimicrobial activity; antioxidant activity; Biosynthesis; Breast cancer; Catalysis; catalytic activity; Chloroauric acid; Cytotoxicity; E coli; Electron microscopy; Field emission; Glycyrrhiza uralensis; Gold; gold nanoparticles; Macrophages; Medicinal plants; Methylene blue; Model testing; Nanoparticles; Pseudomonas aeruginosa; Reagents; Salmonella; Silver; Silver chloride; silver chloride nanoparticles; Silver nitrate; Toxicity; Transmission electron microscopy; X-ray diffraction; Glycyrrhiza uralensis; silver chloride nanoparticles; antimicrobial activity; gold nanoparticles; antioxidant activity; catalytic activity
• ISSN: 2169-1401; 2169-141X
• DOI: 10.1080/21691401.2017.1307213

The current study highlights the rapid biosynthesis of gold nanoparticles (Gu-AuNps) and silver chloride nanoparticles (Gu-AgClNps) by aqueous root extract of Glycyrrhiza uralensis, a medicinal plant. G. uralensis has been reported for anticancer and hepatoprotective effects. The reduction of chloroauric acid and silver nitrate by the Glycyrrhiza root extract prompted the formation of Gu-AuNps and Gu-AgClNps within 4 and 40 min at 80 °C, respectively. The complete reaction did not require supplemental reducing and stabilizing agents, which demonstrated green synthesis. Field emission transmission electron microscopy (FE-TEM) revealed the spherical shape of Gu-AuNps and Gu-AgClNps. X-ray diffraction (XRD) showed face-centred cubic structure of Gu-AuNps and Gu-AgClNps with average crystallite size 12.25 nm and 8.01 nm, respectively. The biosynthesized Gu-AgClNps served as competent antimicrobial agent against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Salmonella enterica. Additionally, Gu-AuNps and Gu-AgClNps were analyzed for their catalytic ability to reduce methylene blue as model test pollutant. Likewise, both nanoparticles possessed free radical scavenging activity against 2,2-diphenyl-1-picrylhydrzyl (DPPH). Moreover, in vitro cytotoxicity in murine macrophage (RAW264.7) and human breast cancer (MCF7) cells were evaluated. Thus, the study proposes a green synthesis of Gu-AuNps and Gu-AgClNps by G. uralensis extract and in vitro biological applications.