Structural characterization and polymorphism analysis of the NS2B-NS3 protease from the 2017 Brazilian circulating strain of Yellow Fever virus

• Published in: Biochimica et biophysica acta. General subjects Vol. 1864; no. 4; p. 129521
• Main Authors: Noske, Gabriela Dias, Gawriljuk, Victor Oliveira, Fernandes, Rafaela Sachetto, Furtado, Nathalia Dias, Bonaldo, Myrna Cristina, Oliva, Glaucius, Godoy, Andre Schutzer
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.04.2020
• Subjects: catalytic activity; crystal structure; Culicidae; fever; Flavivirus; headache; mortality; mutants; nausea; NS2B; NS3; Protease; proteinases; X-ray diffraction; Yellow Fever virus; ORF; TEV; DTT; NS; PNK; Flavivirus; YF; NS3; PDB; UTR; Protease; DENV; DSF; AMC; WT; WNV; SUMO; IMAC; Yellow Fever virus; LIC; YFV; RMSD; TB; ZIKV; NS2B; AU; IPTG; OD600; PCR
• ISSN: 0304-4165; 1872-8006; 1872-8006
• DOI: 10.1016/j.bbagen.2020.129521

The Yellow Fever virus (YFV) is transmitted by mosquitos and causes an infection with symptoms including fever, headaches and nausea. In 20–50% of the cases, the disease may evolve to a visceral stage, reaching high mortality rates. YFV NS2B-NS3 protease has been identified as an important drug target. Herein, we describe the crystal structure of the NS2B-NS3 protease from the 2017 YFV Brazilian circulating strain using X-ray crystallography. Furthermore, we used a combination of biochemical and biophysical assays to characterize the enzyme and investigate the impact of the polymorphisms observed in different YFV circulating strains. Surprisingly, the crystal structure of YFV protease seems to adopt the closed conformation without the presence of a binding partner. Although D88E and K121R mutants exhibited a lower affinity for the substrate, both revealed to be more processive, resulting in a similar catalytic efficiency in relation to the WT protease. Still, both mutants showed an accentuated decrease in stability when compared with the WT. The crystal structure of YFV NS2B-NS3 in closed conformation might be an important tool for the development of new drugs, as well as understanding the activation mechanism of viral proteases. Biochemical analyses indicate that the NS2B-NS3 protease of the circulating strain of YFV is more stable than previous strains. The YFV NS2B-NS3 protease is the first flaviviral structure described in its closed conformation when in a free form, implying that external factors might induce the activation of the enzyme. [Display omitted] •Structure of Yellow fever virus NS2B-NS3 protease is elucidated•The structure was crystallized in its closed conformation without the presence of a ligand•Protease from the new circulating strain has increased activity and stability when compared with previous strains