A new polysaccharide from leaves of Sabia parviflora

• Published in: Natural product research Vol. 38; no. 8; pp. 1302 - 1310
• Main Authors: Wu, Sijing, Li, Hui, Li, Manshu, Xu, Bang, Liu, Chengxiong, Zou, Kun
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 17.04.2024; Taylor & Francis Ltd
• Subjects: Crystals; extraction and isolation; Hepatitis; hepatoprotective activity; Liver; Magnetic resonance spectroscopy; Molecular weight; NMR spectroscopy; Plant Leaves - chemistry; polysaccharide; Polysaccharides; Polysaccharides - analysis; Polysaccharides - pharmacology; PTP1B inhibitory activity; Sabia parviflora; structure identification; Sabia parviflora; hepatoprotective activity; PTP1B inhibitory activity; polysaccharide; extraction and isolation; structure identification
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2022.2139698

A new polysaccharide (SPT1) was isolated from Sabia parviflora Wall. ex Roxb., and the structure was identified by GPC, 1D and 2D NMR spectroscopy, SEM and AFM. The results showed that the average molecular weight (Mn) of SPT1 was 4.057 × 10 3  Da, and it was composed of α-glucose with a connection mode of 1→6. The SEM showed that the particle size of SPT1 was 1-200 μm and there were small gaps between the crystals. SPT1 was mainly spherical aggregates in AFM, each aggregate was 0.550-0.983 μm long, 1.059-2.275 μm wide and 208-450 nm high. Furthermore, its liver-protective and PTP1B inhibitory activities were evaluated, and the results showed that SPT1 exhibited moderate effects of liver-protective and PTP1B inhibitory activity. The above results provided experimental evidence for the folk application of S. parviflora in the treatment of hepatitis.