Effect of protein corona magnetite nanoparticles derived from bread in vitro digestion on Caco-2 cells morphology and uptake

• Published in: The international journal of biochemistry & cell biology Vol. 75; pp. 212 - 222
• Main Authors: Di Silvio, Desirè, Rigby, Neil, Bajka, Balazs, Mackie, Alan, Baldelli Bombelli, Francesca
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01.06.2016
• Subjects: Biomimetic Materials - metabolism; blood serum; Body Fluids - metabolism; Bread; breads; Caco-2; Cell uptake; cellular microenvironment; confocal microscopy; Digestion; food matrix; Gastrointestinal digestion; human cell lines; humans; in vitro digestion; magnetite; Magnetite Nanoparticles - chemistry; Nanoparticle; nanoparticles; Particle Size; protein composition; Protein corona; Protein Corona - chemistry; Protein Corona - metabolism; Protein Transport; proteins; sucrose; ultracentrifugation; Gastrointestinal digestion; Cell uptake; NP; GI; CF; Nanoparticle; SSF; UC; PMAO; Bread; SIF; PC; SGF; Caco-2; FA; SFM; Protein corona
• ISSN: 1357-2725; 1878-5875; 1878-5875
• DOI: 10.1016/j.biocel.2015.10.019

•Digestive protein corona nanoparticles are isolated by sucrose gradient ultracentrifugation.•Proteins enriched in the corona are partially protected during digestion.•PC formation enhances uptake of magnetite nanoparticle in Caco-2 cells.•Gastric and duodenal PCs induce different alterations in Caco-2 morphology. Nanoparticles (NPs) in biological fluids immediately interact with proteins forming a biomolecular corona (PC) that imparts their biological identity. While several studies on the formation of the PC in human plasma have been reported, the PC of orally administrated NPs has been less investigated, mostly in the presence of a food matrix. In fact, food matrixes when digested are subject of several dynamic changes that will certainly affect the PC formed on the NPs. The lack of studies on this topic is clearly related to the difficulty in isolating representative PC NPs from such a complex environment. In this work magnetite NPs were added to in vitro simulated digestion simultaneously with bread and PC NPs were isolated after gastric and duodenal phases by sucrose gradient ultracentrifugation (UC). The PC NPs were characterized in terms of size and protein composition. Translocation studies were then performed on Caco-2 monolayers in a serum free environment and cell morphology was characterized by confocal microscopy. PC NPs isolated from gastric and duodenal phases were different in size, surface charge and protein corona composition. NP cellular uptake was enhanced by the digestive PC inducing morphology changes in the cell monolayer. Overall, in this work we were able to isolate PC NPs from digested fluids in the presence of a food matrix and study their biological response on Caco-2 cells.