Effect of Addition of Silymarin to Renin-Angiotensin System Inhibitors on Proteinuria in Type 2 Diabetic Patients With Overt Nephropathy: A Randomized, Double-Blind, Placebo-Controlled Trial

• Published in: American journal of kidney diseases Vol. 60; no. 6; pp. 896 - 903
• Main Authors: Fallahzadeh, Mohammad Kazem, MD, Dormanesh, Banafshe, MD, Sagheb, Mohammad Mahdi, MD, Roozbeh, Jamshid, MD, Vessal, Ghazal, PharmD, PhD, Pakfetrat, Maryam, MD, Daneshbod, Yahya, MD, Kamali-Sarvestani, Eskandar, PhD, Lankarani, Kamran B., MD
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.12.2012; Elsevier
• Subjects: Adult; Aged; Angiotensin II Type 1 Receptor Blockers - administration & dosage; Angiotensin-Converting Enzyme Inhibitors - administration & dosage; Antioxidants - administration & dosage; Biological and medical sciences; Diabetes Mellitus, Type 2 - drug therapy; Diabetes Mellitus, Type 2 - epidemiology; Diabetes Mellitus, Type 2 - metabolism; Diabetes. Impaired glucose tolerance; Diabetic Nephropathies - drug therapy; Diabetic Nephropathies - epidemiology; Diabetic Nephropathies - metabolism; Diabetic nephropathy; Double-Blind Method; Drug Therapy, Combination; Endocrine pancreas. Apud cells (diseases); Endocrinopathies; Etiopathogenesis. Screening. Investigations. Target tissue resistance; Female; flavonoids; Humans; inflammation; Male; Medical sciences; Middle Aged; milk thistle; Nephrology; Nephrology. Urinary tract diseases; oxidative stress; Proteinuria - drug therapy; Proteinuria - epidemiology; Proteinuria - metabolism; Renin-Angiotensin System - drug effects; Renin-Angiotensin System - physiology; silymarin; Silymarin - administration & dosage; Treatment Outcome; Urinary system involvement in other diseases. Miscellaneous; Urinary tract. Prostate gland; Diabetic nephropathy; silymarin; flavonoids; inflammation; milk thistle; oxidative stress; Endocrinopathy; Type 2 diabetes; Oxidative stress; Nephrology; Flavonoid; Urology; Polyphenol; Milk; Kidney disease; Human; Urinary system disease; Metabolic diseases; Inflammation; Randomized controlled trial; Concomitant disease; Renin angiotensin system; Silibinin; Phenols; Double blind study; Inhibitor; Proteinuria
• ISSN: 0272-6386; 1523-6838
• DOI: 10.1053/j.ajkd.2012.06.005

Background A large proportion of patients with type 2 diabetes mellitus have diabetic nephropathy. Despite current therapies including renin-angiotensin system inhibitors, diabetic nephropathy progresses to end-stage renal disease in most of these patients. Therefore, there is an urgent need to find new treatments for such patients. The aim of this study was to evaluate the efficacy of silymarin, an herbal drug with antioxidant and anti-inflammatory properties, in preventing the progression of diabetic nephropathy. Study Design Randomized, double-blind, placebo-controlled, 2-arm parallel trial. Setting & Participants 60 patients with type 2 diabetes with macroalbuminuria (urinary albumin excretion >300 mg/24 h) despite treatment with the maximum dose of a renin-angiotensin system inhibitor for more than 6 months and estimated glomerular filtration rate >30 mL/min/1.73 m2. Intervention Patients were randomly assigned to 2 equal groups to receive three 140-mg tablets of silymarin or 3 tablets of placebo daily for 3 months. Outcomes The primary outcome was absolute change in urinary albumin-creatinine ratio (UACR) from baseline to the end of the treatment phase. Measurements UACR and urinary and serum levels of TNF-α (tumor necrosis factor α; an inflammatory marker), malondialdehyde (MDA; an oxidative stress marker), and TGFβ (transforming growth factor β; a marker of fibrosis) at baseline and the end of the treatment phase. Results Although UACR decreased in both groups, this decrement was significantly higher in the silymarin compared with the placebo group; mean difference in change in UACR between the 2 groups was −347 (95% CI, −690 to −4) mg/g. Urinary levels of TNF-α and urinary and serum levels of MDA also decreased significantly in the silymarin compared with the placebo group. Limitations Small sample size and short duration of the treatment phase. Conclusions Silymarin reduces urinary excretion of albumin, TNF-α, and MDA in patients with diabetic nephropathy and may be considered as a novel addition to the anti–diabetic nephropathy armamentarium.