Antioxidant Effect of Salvianolic Acid B on Hippocampal CA1 Neurons in Mice with Cerebral Ischemia and Reperfusion Injury

• Published in: Chinese journal of integrative medicine Vol. 21; no. 7; pp. 516 - 522
• Main Author: 蒋玉凤 刘智勤 崔巍 张文通 龚家培 王玺玫 张颖 杨美娟
• Format: Journal Article
• Language: English
• Published: Beijing Chinese Association of Traditional and Western Medicine 01.07.2015
• Subjects: Animals; Antioxidants - pharmacology; Antioxidants - therapeutic use; bcl-2-Associated X Protein - metabolism; Bcl-2蛋白; Benzofurans - chemistry; Benzofurans - pharmacology; Benzofurans - therapeutic use; Brain Ischemia - complications; Brain Ischemia - drug therapy; CA1 Region, Hippocampal - pathology; Cell Count; Immunohistochemistry; Male; Malondialdehyde - metabolism; Medicine; Medicine & Public Health; Mice; Neurons - drug effects; Neurons - pathology; Nitric Oxide Synthase - metabolism; Original Article; Reperfusion Injury - complications; Reperfusion Injury - drug therapy; Superoxide Dismutase - metabolism; 丹酚酸B; 免疫组织化学染色; 抗氧化作用; 海马CA1区; 神经元; 缺血再灌注损伤; 鼠脑; neuron; apoptosis; ischemia-reperfusion; hippocampus; Salvianolic acid B
• ISSN: 1672-0415; 1993-0402; 1993-0402
• DOI: 10.1007/s11655-014-1791-1

Objetive： TO investigate the neuroprotective effects and underlying mechanisms of salvianolic acid B （Sal B） extracted from Salvia miltiorrhiza on hippocampal CA1 neurons in mice with cerebral ischemia reperfusion injury. Methods： Forty male National Institute of Health （NIH） mice were randomly divided into 4 groups with 10 animals each, including the sham group, the model group, the SalB group （SalB 22.5 mg/kg） and the nimodipine （Nim） group （Nim 1 mg/kg）. A mouse model of cerebral ischemia and reperfusion injury was established by bilateral carotid artery occlusion for 30 rain followed by 24-h reperfusion. The malondialdehyde （MDA） content, the nitric oxide synthase （NOS） activity, the superoxide dismutase （SOD） activity and total anti- oxidant capability （T-AOC） of the pallium were determined by biochemistry methods. The morphologic changes and Bcl-2 and Bax protein expression in hippocampal CA1 neurons were observed by using hematoxylin- eosin staining and immunohistochemistry staining, respectively. Results： In the SalB group, the MDA content and the NOS activity of the pallium in cerebral ischemia-reperfusion mice significantly decreased and the SOD activity and the T-AOC significantly increased, as compared with the model group （P〈0.05 or P〈0.01）. The SalB treatment also rescued neuronal loss （P〈0.01） in the hippocampal CA1 region, strongly promoted Bcl-2 protein expression （P〈0.01） and inhibited Bax protein expression （P〈0.05）. Conclusions： SalB increases the level of antioxidant substances and decreases free radicals production. Moreover, it also improves Bcl-2 expression and reduces Bax expression. SalB may exert the neuroprotective effect through mitochondria-dependent pathway on hippocampal CA1 neurons in mice with cerebral ischemia and reperfusion injury and suggested that SalB represents a promising candidate for the prevention and treatment of ischemic cerebrovascular disease.