Antileishmanial and antioxidant potential of fractions and isolated compounds from Nectandra cuspidata

• Published in: Natural product research Vol. 32; no. 23; pp. 2825 - 2828
• Main Authors: Ferreira, Lilian dos Anjos Oliveira, Oliveira, Mariana Maciel de, Faleiro, Flávia Lais, Scariot, Débora Botura, Boeing, Joana Schuelter, Visentainer, Jesuí Vergílio, Romagnolo, Mariza Barion, Nakamura, Celso Vataru, Truiti, Maria da Conceição Torrado
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 02.12.2018; Taylor & Francis Ltd
• Subjects: Acetic acid; Animals; Antioxidants; Antioxidants - isolation & purification; Antioxidants - pharmacology; Antiprotozoal Agents - chemistry; Antiprotozoal Agents - isolation & purification; Antiprotozoal Agents - pharmacology; Butylated hydroxytoluene; Cell Line; Cytotoxicity; Epicatechin; Ethyl acetate; lauraceae; Lauraceae - chemistry; Leishmania; Leishmania - drug effects; Leishmaniasis; Macrophages; Macrophages - drug effects; medicinal plant; Mice; Nectandra; oxidative stress; Phenols - analysis; Plant Extracts - chemistry; Plant Extracts - pharmacology; Quercetin; Toxicity; Viability; lauraceae; medicinal plant; Leishmaniasis; oxidative stress
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2017.1378214

The effects on the viability of promastigote and amastigote forms of Leishmania (Leishmania) amazonensis and citotoxicity in J774.A1 macrophages of ethanolic extract, fractions and isolated compounds that were obtained from the leaves of Nectandra cuspidata were determined. The total phenolics content (TP) and antioxidant activity were assessed using different assays. The ethyl acetate fraction (EAF) had the best antiproliferative activity (amastigote IC 50 : 4.42 ± 0.60 μg/mL, promastigote IC 50 : 33.33 ± 0.78) and presented no cytotoxicity at the highest concentration tested (1000 μg/mL). The EAF also had the greatest antioxidant capacity, which was comparable to butylated hydroxytoluene and quercetin (DPPH IC 50 6.54 ± 0.10 μg/mL, ABTS 4.75 ± 0.11 mmol ET/g; FRAP 2.37 ± 0.03 mmol ET/g; ORAC = 33.52 ± 1.99 mmol ET/g; TP 387.11 ± 0.25 mg GAE/g). Vitexin, epicatechin and isovitexin isolated from EAF contribute to both activities.