Evaluation of potential genotoxicity of stannous chloride: inactivation, filamentation and lysogenic induction of escherichia coli

• Published in: Food and chemical toxicology Vol. 32; no. 5; pp. 477 - 479
• Main Authors: Bernardo-Filho, M., da Conceicão Cunha, M., de Oliveira Valsa, J., Caldeira de Araujo, A., Campos Pereira da Silva, F., de Souza da Fonseca, A.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.05.1994; New York, NY Elsevier Science
• Subjects: Biological and medical sciences; Chemical and industrial products toxicology. Toxic occupational diseases; chlorides; DNA Repair - genetics; Escherichia coli; Escherichia coli - drug effects; Escherichia coli - genetics; Escherichia coli - growth & development; genes; inactivation; Lysogeny - drug effects; Medical sciences; Metals and various inorganic compounds; Mutagenicity Tests - methods; Mutagens - toxicity; Tin Compounds - toxicity; toxicity; Toxicology; LB; Carcinogen; Mutagenicity testing; Toxicity; DNA; Escherichia coli; Tin II Chlorides; Bacteria; Mutagen; Enterobacteriaceae; Heavy metal
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/0278-6915(94)90045-0

Because of the importance of stannous chloride in various fields of human endeavour, the potential genotoxicity of this reducing agent was evaluated by measurement of either the inactivation or the induction of SOS responses in bacteria. Escherichia coli strains used in this work (wild type, uvr A, trecA, lexA and uvr A recA) were treated with stannous chloride; the wild type was found to be the most resistant and the double mutant, the most sensitive strain. As these strains present mutations on specific genes for the repair of DNA, stannous chloride would appear to be capable of inducing and/or producing lesions in DNA and, thus, can be considered to be a potential genotoxic agent. This capability was confirmed by the lysogenic induction of E. coli K 12 (λ) (Inductest) and by microscopic observation of E. coli B filamentation.