Optimization of the sensitivity of high-performance liquid chromatography in the detection of heterocyclic aromatic amine mutagens

• Published in: Food and chemical toxicology Vol. 30; no. 6; pp. 491 - 498
• Main Authors: Gross, G.A., Grüter, A., Heyland, S.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.06.1992; New York, NY Elsevier Science
• Subjects: amines; Amines - analysis; Amines - isolation & purification; aromatic compounds; Biological and medical sciences; Chemical mutagenesis; Chromatography, High Pressure Liquid - standards; Chromatography, Ion Exchange; flavorings; Food Analysis - methods; high performance liquid chromatography; Hot Temperature; Meat Products - analysis; Medical sciences; mutagenicity; Mutagens - analysis; Mutagens - isolation & purification; Sensitivity and Specificity; Toxicology; MeIQ; harman; norharman; 4,8-DiMeIQx; IQ; Trp-P-2; HAAs; Trp-P-1; PRS; AαC; Glu-P-2; MeIQx; MeOH; TSK CM; PhIP; Glu-P-1; Assay; Bakery product; Amine; Analytical method; HPLC chromatography; Aromatic compound; Extraction; Mutagen; Heterocyclic compound; Food
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/0278-6915(92)90100-Y

Solid-phase extraction with the weak cation-exchange resin Fractogel TSK CM650(S) (TSK CM) was used to optimize the sensitivity and chromatographic resolution by HPLC of the mutagenic heterocyclic aromatic amines MeIQx, 4,8-DiMeIQx, IQ, MeIQ, PhIP, Trp-P-1, Trp-P-2, amino-α-carboline and the co-mutagens norharman and harman. The clean-up using cation exchange was applied after purification on Extrelut and propylsulphonic acid—silica gel cartridges (PRS tandem purification). Only the clean-up with TSK CM enabled low detection limits of the mutagenic compounds to be achieved (in the order of ng heterocyclic amines/g experimentally overheated process-flavour sample).