Involvement of a guanine-nucleotide-binding component in membrane IgM- stimulated phosphoinositide breakdown

• Published in: The Journal of immunology (1950) Vol. 139; no. 11; pp. 3604 - 3613
• Main Authors: Gold, MR, Jakway, JP, DeFranco, AL
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Am Assoc Immnol 01.12.1987; American Association of Immunologists
• Subjects: Adenylate Cyclase Toxin; Animals; Applied sciences; B-Lymphocytes - drug effects; B-Lymphocytes - immunology; B-Lymphocytes - physiology; Calcium - metabolism; Cell Line; cell membranes; Cholera Toxin - pharmacology; Exact sciences and technology; GTP-Binding Proteins - physiology; guanine nucleotide-binding protein; Guanosine 5'-O-(3-Thiotriphosphate); Guanosine Triphosphate - analogs & derivatives; Guanosine Triphosphate - metabolism; immunoglobulin M; Immunoglobulin M - immunology; lymphocytes B; Mice; Other techniques and industries; Pertussis Toxin; phosphatidylinositol 4,5-bisphosphate; Phosphatidylinositols - metabolism; phospholipase; Receptors, Antigen, B-Cell - immunology; Thionucleotides - metabolism; Tumor Cells, Cultured - drug effects; Tumor Cells, Cultured - immunology; Tumor Cells, Cultured - physiology; Virulence Factors, Bordetella - pharmacology

Cross-linking of membrane immunoglobulin, the B cell receptor for antigen, activates the phosphoinositide signal transduction pathway. The initial event in this pathway is the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PtdInsP2) by phospholipase C. This reaction yields two intracellular se...