New molecular markers for phlebotomine sand flies

• Published in: International journal for parasitology Vol. 31; no. 5-6; pp. 635 - 639
• Main Authors: PEIXOTO, A. A, GOMES, C. A, DE AMORETTY, P. R, LINS, R. M. M. A, MEIRELES-FILHO, A. C. A, DE SOUZA, N. A, KYRIACOU, C. P
• Format: Conference Proceeding Journal Article
• Language: English
• Published: Oxford Elsevier Science 01.05.2001
• Subjects: Amino Acid Sequence; Animals; Biological and medical sciences; cacophony gene; Codon - chemistry; DNA - genetics; DNA - isolation & purification; Drosophila; Drosophila - genetics; Fundamental and applied biological sciences. Psychology; Genetic Markers - genetics; Leishmania; Lutzomyia longipalpis; Medically important nuisances and vectors, pests of stored products and materials: population survey and control; Molecular Sequence Data; period gene; Phylogeny; Polymerase Chain Reaction; Psychodidae; Psychodidae - chemistry; Psychodidae - classification; Psychodidae - genetics; Sequence Alignment; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Sexual Behavior, Animal - physiology; Vectors. Intermediate hosts; Kinetoplastida; Genetic mapping; Protozoa; Host parasite relation; Protozoal disease; Genetic marker; Nucleotide sequence; Insecta; Biological evolution; Psychodidae; Homology; Parasitosis; Drosophila virilis; Leishmaniasis; Population genetics; Drosophilidae; Infection; Arthropoda; Leishmania; Invertebrata; Vector; Diptera; Lutzomyia longipalpis; Aminoacid sequence
• ISSN: 0020-7519; 1879-0135
• DOI: 10.1016/S0020-7519(01)00149-7

Using degenerate-primers PCR we isolated and sequenced fragments from the sand fly Lutzomyia longipalpis homologous to two behavioural genes in Drosophila, cacophony and period. In addition we identified a number of other gene fragments that show homology to genes previously cloned in Drosophila. A codon usage table for L. longipalpis based on these and other genes was calculated. These new molecular markers will be useful in population genetics and evolutionary studies in phlebotomine sand flies and in establishing a preliminary genetic map in these important leishmaniasis vectors.