Identification, Sequencing, and Molecular Analysis of RNA2 of Artichoke Italian Latent Virus Isolates from Known Hosts and a New Host Plant Species

• Published in: Viruses Vol. 15; no. 11; p. 2170
• Main Authors: Elbeaino, Toufic, Ben Slimen, Amani, Belgacem, Imen, Mnari-Hattab, Monia, Spanò, Roberta, Digiaro, Michele, Abdelkhalek, Ahmed
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 28.10.2023
• Subjects: Acids; Amino acid sequence; Artichokes; Cloning; Cynara scolymus - genetics; Diseases and pests; Flowers & plants; Genetic aspects; Genomes; Host plants; Hybridization; Identification and classification; Italy; Methods; Nepovirus; Nucleotide sequence; phylogenetic and recombination analyses; Phylogenetics; Phylogeny; Plant viruses; Proteins; Recombination; RNA sequencing; RNA, Viral - chemistry; RNA, Viral - genetics; RNA2 sequences; Sequence Analysis, DNA; Virus diseases of plants; Viruses; Italy; nepovirus; RNA2 sequences; phylogenetic and recombination analyses
• ISSN: 1999-4915; 1999-4915
• DOI: 10.3390/v15112170

Despite its first description in 1977 and numerous reports of its presence in various plant species in many countries, the molecular information available in GenBank for artichoke Italian latent virus (AILV) is still limited to a single complete genome sequence (RNA1 and 2) of a grapevine isolate (AILV-V) and a partial portion of the RNA2 sequence from an isolate of unknown origin and host. Here, we report the results of molecular analyses conducted on the RNA2 of some AILV isolates, sequenced for the first time in this study, together with the first-time identification of AILV in a new host plant species, namely chard ( subsp. vulgaris), associated with vein clearing and mottling symptoms on leaves. The different AILV isolates sequenced were from artichoke (AILV-C), gladiolus (AILV-G), Sonchus (AILV-S), and chard (AILV-B). At the molecular level, the sequencing results of the RNA2 segments showed that AILV-C, AILV-G, AILV-S, and AILV-B had a length of 4629 nt (excluding the 3' terminal polyA tail), which is one nt shorter than that of the AILV-V reported in GenBank. A comparison of the RNA2 coding region sequences of all the isolates showed that AILV-V was the most divergent isolate, with the lowest sequence identities of 83.2% at the nucleotide level and 84.7% at the amino acid level. Putative intra-species sequence recombination sites were predicted among the AILV isolates, mainly involving the genomes of AILV-V, AILV-C, and AILV-B. This study adds insights into the variability of AILV and the occurrence of recombination that may condition plant infection.