Molecular Cloning and Characterization of a Novel Lectin Gene from Zephyranthes candida

A new lectin gene was cloned from Zephyranthes candida by using RACE-PCR. The full-length cDNA of Zephyranthes candida agglutinin (ZCA) was 647 bp and contained a 477 bp open reading frame encoding a 159 amino acid protein. Zephyranthes candida lectin gene was found to encode a precursor lectin with...

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Published inDNA sequence Vol. 14; no. 3; pp. 163 - 167
Main Authors Pang, Yong-Zhen, Shen, Guo-An, Liao, Zhi-Hua, Yao, Jian-Hong, Fei, Jiong, Sun, Xiao-Fen, Tan, Feng, Tang, Ke-Xuan
Format Journal Article
LanguageEnglish
Published England Taylor & Francis 01.06.2003
Subjects
Amino Acid Sequence
Base Sequence
Candida
cDNA
DNA Primers
DNA, Complementary - genetics
Lectin
Lectins - genetics
Liliaceae - genetics
Models, Molecular
Molecular Sequence Data
RACE
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology
Zephyranthes candida
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Summary:A new lectin gene was cloned from Zephyranthes candida by using RACE-PCR. The full-length cDNA of Zephyranthes candida agglutinin (ZCA) was 647 bp and contained a 477 bp open reading frame encoding a 159 amino acid protein. Zephyranthes candida lectin gene was found to encode a precursor lectin with signal peptide and had extensive homology with those of other plant lectins. Molecular modeling of ZCA indicated that the three-dimensional structure of ZCA strongly resembles that of the snowdrop lectin, implying ZCA may have the similar insecticidal functions with GNA.
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ISSN:1042-5179
1029-2365
DOI:10.1080/1042517031000089450