The MTCY428.08 gene of Mycobacterium tuberculosis codes for NAD+ synthetase

The product of the MTCY428.08 gene of Mycobacterium tuberculosis shows sequence homology with several NAD+ synthetases. The MTCY428.08 gene was cloned into the expression vectors pGEX-4T-1 and pET-15b. Expression in Escherichia coli led to overproduction of glutathione S-transferase fused and His6-t...

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Published inJournal of bacteriology Vol. 180; no. 12; pp. 3218 - 3221
Main Authors Cantoni, R, Branzoni, M, Labò, M, Rizzi, M, Riccardi, G
Format Journal Article
LanguageEnglish
Published United States American Society for Microbiology 01.06.1998
SeriesNote
Subjects
Amino Acid Sequence
Bacteria
Bacteriology
Carbon-Nitrogen Ligases with Glutamine as Amide-N-Donor - genetics
Carbon-Nitrogen Ligases with Glutamine as Amide-N-Donor - metabolism
Cloning
Cloning, Molecular
Enzymes
Escherichia coli - genetics
Genes
Genes, Bacterial
Genetics
Genetics and Molecular Biology
Microbiology
Molecular Sequence Data
Mycobacterium tuberculosis - enzymology
Mycobacterium tuberculosis - genetics
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - metabolism
Sequence Homology, Amino Acid
Tuberculosis
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Summary:The product of the MTCY428.08 gene of Mycobacterium tuberculosis shows sequence homology with several NAD+ synthetases. The MTCY428.08 gene was cloned into the expression vectors pGEX-4T-1 and pET-15b. Expression in Escherichia coli led to overproduction of glutathione S-transferase fused and His6-tagged gene products, which were enzymatically assayed for NAD synthetase activity. Our results demonstrate that the MTCY428.08 gene of M. tuberculosis is the structural gene for NAD+ synthetase.
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Corresponding author. Mailing address: Dipartimento di Genetica e Microbiologia, Università di Pavia, via Abbiategrasso 207, 27100 Pavia, Italy. Phone: (39) 382 505 574. Fax: (39) 382 52 84 96. E-mail: Riccardi@ipvgen.unipv.it.
ISSN:0021-9193
1098-5530
DOI:10.1128/jb.180.12.3218-3221.1998