Production of Human Monoclonal Antibody to X31 Influenza Virus Nucleoprotein

• Published in: Journal of general virology Vol. 64; no. 3; pp. 697 - 700
• Main Authors: Crawford, D. H, Callard, R. E, Muggeridge, M. I, Mitchell, D. M, Zanders, E. D, Beverley, P. C. L
• Format: Journal Article
• Language: English
• Published: England Soc General Microbiol 01.03.1983
• Subjects: Antibodies, Monoclonal - immunology; Antibodies, Viral - immunology; Antigens, Viral - immunology; B-Lymphocytes - immunology; Cell Line; Herpesvirus 4, Human - immunology; Humans; Immunoglobulin G - immunology; Influenza A virus - immunology; influenza virus; Lymphocyte Activation; Nucleoproteins - immunology; Ribonucleoproteins - immunology; Viral Proteins - immunology
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/0022-1317-64-3-697

1 Department of Haematology and ICRF Human Tumour Immunology Group University College Hospital Medical School University Street, London WC1E 6JJ, U.K. 2 Immunology Unit, Department of Bacteriology University of Sydney Medical School, University of Sydney, New South Wales 2006, Australia 3 Department of Virology, National Institute for Medical Research The Ridgeway, Mill Hill, London NW7 1AA, U.K. In vitro stimulation of human peripheral blood mononuclear cells with X31 influenza virus antigen has been used to enrich for specific anti-X31 antibody-producing cells. Following Epstein—Barr virus transformation of these stimulated cells, a cell line which produces human antibody to X31 virus was derived and subsequently cloned. The cloned cells secrete an IgG1 antibody which is directed against the nucleoprotein of A type influenza virus. Culture supernatants contain 10 to 20 µg/ml of specific antibody which is now used as a standard for the ELISA assay used in our laboratory to detect antibodies to influenza virus. Keywords: human monoclonal antibody, influenza virus X31, nucleoprotein Received 28 July 1982; accepted 7 October 1982.