Studies on the interfacial behavior of DPPC/DPPG mixed monolayers in the presence of fluoxetine

• Published in: Journal of molecular modeling Vol. 26; no. 7; p. 167
• Main Authors: Xie, Bin, Hao, Changchun, Zhang, Ziyi, Sun, Runguang
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 08.06.2020; Springer Nature B.V
• Subjects: 1,2-Dipalmitoylphosphatidylcholine - chemistry; Atomic force microscopy; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Compressibility; Computer Appl. in Life Sciences; Computer Applications in Chemistry; Fluoxetine - chemistry; Homogeneity; Langmuir-Blodgett films; Lipids; Membranes, Artificial; Microscopy, Atomic Force; Miscibility; Models, Molecular; Molecular Medicine; Monolayers; Morphology; Original Paper; Phosphatidylglycerols - chemistry; Pressure; Serotonin Uptake Inhibitors - chemistry; Static Electricity; Surface Properties; Surface stability; Theoretical and Computational Chemistry; Mixed monolayers; Interfacial behavior; Atomic force microscope; Fluoxetine; Electrostatic interaction
• ISSN: 1610-2940; 0948-5023
• DOI: 10.1007/s00894-020-04433-1

In this study, the interfacial behavior of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine/1,2-dipalmitoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (DPPC/DPPG) mixed monolayers with fluoxetine (FLX) in the subphase was investigated by a combination of the Langmuir-Blodgett technique and atomic force microscopy (AFM). It was found that DPPC/DPPG mixed monolayers showed different interfacial behaviors before and after addition of FLX in the subphase. The electrostatic interaction between FLX and lipids molecules destroys the homogeneity of the mixed monolayers and changes the arrangement of lipids molecules at the interface after addition of FLX in the subphase, thereby leading to an increase of compressibility and miscibility and a decrease in the stability of the mixed monolayers. The surface morphology of the mixed monolayers observed by AFM was different between without and with FLX in the subphase, indicating the penetration of FLX into the mixed monolayers. The present study has provided detailed information for further understanding the interactions of drugs with membrane lipids in other lipid monolayers.