Decreased Hepatic and Serum Levels of IL-10 Concur with Increased Lobular Inflammation in Morbidly Obese Patients

• Published in: Medicina (Kaunas, Lithuania) Vol. 60; no. 6; p. 862
• Main Authors: Solleiro-Villavicencio, Helena, Méndez-García, Lucía Angélica, Ocampo-Aguilera, Nydia A, Baltazar-Pérez, Itzel, Arreola-Miranda, José A, Aguayo-Guerrero, José A, Alfaro-Cruz, Ana, González-Chávez, Antonio, Fonseca-Sánchez, Miguel A, Fragoso, José Manuel, Escobedo, Galileo
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 25.05.2024; MDPI
• Subjects: Adult; B cells; Body mass index; Creatinine; Cytokines; Diabetes; Female; Hemoglobin; High density lipoprotein; Humans; IL-10; Inflammation; Inflammation - blood; Interleukin-10 - analysis; Interleukin-10 - blood; Laboratories; Lipids; Liver; Liver - metabolism; Liver - pathology; liver biopsy; Liver cancer; Liver diseases; lobular inflammation; Male; Metabolism; Middle Aged; morbid obesity; NASH; Non-alcoholic Fatty Liver Disease - blood; Non-alcoholic Fatty Liver Disease - complications; Obesity; Obesity, Morbid - blood; Obesity, Morbid - complications; Prospective Studies; Proteins; simple steatosis; Surgery; Triglycerides; Type 2 diabetes; Uric acid; Variance analysis; Mexico; Minneapolis Minnesota; United States > US; Germany; morbid obesity; NASH; liver biopsy; lobular inflammation; IL-10; simple steatosis
• ISSN: 1648-9144; 1010-660X; 1648-9144
• DOI: 10.3390/medicina60060862

: Non-alcoholic fatty liver disease (NAFLD) is associated with obesity and ranges from simple steatosis to non-alcoholic steatohepatitis (NASH), fibrosis, cirrhosis, and hepatocellular carcinoma. Accumulating evidence in animal models suggests that loss of interleukin-10 (IL-10) anti-inflammatory actions might contribute to lobular inflammation, considered one of the first steps toward NASH development. However, the role of IL-10 in lobular inflammation remains poorly explored in humans. We examined mRNA and protein levels of IL-10 in liver biopsies and serum samples from morbidly obese patients, investigating the relationship between IL-10 and lobular inflammation degree. : We prospectively enrolled morbidly obese patients of both sexes, assessing the lobular inflammation grade by the Brunt scoring system to categorize participants into mild ( = 7), moderate ( = 19), or severe ( = 13) lobular inflammation groups. We quantified the hepatic mRNA expression of IL-10 by quantitative polymerase chain reaction and protein IL-10 levels in liver and serum samples by Luminex Assay. We estimated statistical differences by one-way analysis of variance (ANOVA) and Tukey's multiple comparison test. : The hepatic expression of IL-10 significantly diminished in patients with severe lobular inflammation compared with the moderate lobular inflammation group ( = 0.01). The hepatic IL-10 protein levels decreased in patients with moderate or severe lobular inflammation compared with the mild lobular inflammation group ( = 0.008 and = 0.0008, respectively). In circulation, IL-10 also significantly decreased in subjects with moderate or severe lobular inflammation compared with the mild lobular inflammation group ( = 0.005 and < 0.0001, respectively). : In liver biopsies and serum samples of morbidly obese patients, the protein levels of IL-10 progressively decrease as lobular inflammation increases, supporting the hypothesis that lobular inflammation develops because of the loss of the IL-10-mediated anti-inflammatory counterbalance.