Anatomically distinct fibroblast subsets determine skin autoimmune patterns

• Published in: Nature (London) Vol. 601; no. 7891; pp. 118 - 124
• Main Authors: Xu, Zijian, Chen, Daoming, Hu, Yucheng, Jiang, Kaiju, Huang, Huanwei, Du, Yingxue, Wu, Wenbo, Wang, Jiawen, Sui, Jianhua, Wang, Wenhui, Zhang, Long, Li, Shuli, Li, Chunying, Yang, Yong, Chang, Jianmin, Chen, Ting
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 06.01.2022; Nature Publishing Group
• Subjects: 13/31; 13/89; 14/19; 38/91; 631/136/756; 631/250/38; 64/60; Adolescent; Adult; Animal models; Animals; Antigens; Autoimmune diseases; Autoimmune Diseases - immunology; Autoimmune Diseases - pathology; Barriers; CD8 antigen; CD8-Positive T-Lymphocytes - immunology; Cell activation; Chemokine CXCL10 - immunology; Chemokine CXCL9 - immunology; Chemokines; Child; Cytotoxicity; Disease Models, Animal; Experiments; Female; Fibroblasts; Fibroblasts - immunology; Fibroblasts - pathology; Humanities and Social Sciences; Humans; Immune system; Immunology; Interferon; Interferon-gamma - immunology; Lesions; Lymphocytes; Lymphocytes T; Male; Melanocytes; Melanocytes - immunology; Melanocytes - pathology; Melanoma; Mesenchyme; Mice; Middle Aged; multidisciplinary; Paracrine Communication; Patients; RNA-Seq; Science; Science (multidisciplinary); Single-Cell Analysis; Skin; Skin - immunology; Skin - pathology; Skin diseases; Stromal Cells - immunology; Subpopulations; T-Lymphocytes, Cytotoxic - immunology; Therapeutic targets; Vitiligo; Vitiligo - immunology; Vitiligo - pathology; Young Adult; γ-Interferon
• ISSN: 0028-0836; 1476-4687; 1476-4687
• DOI: 10.1038/s41586-021-04221-8

The skin serves as a physical barrier and an immunological interface that protects the body from the external environment 1 – 3 . Aberrant activation of immune cells can induce common skin autoimmune diseases such as vitiligo, which are often characterized by bilateral symmetric lesions in certain anatomic regions of the body 4 – 6 . Understanding what orchestrates the activities of cutaneous immune cells at an organ level is necessary for the treatment of autoimmune diseases. Here we identify subsets of dermal fibroblasts that are responsible for driving patterned autoimmune activity, by using a robust mouse model of vitiligo that is based on the activation of endogenous auto-reactive CD8 + T cells that target epidermal melanocytes. Using a combination of single-cell analysis of skin samples from patients with vitiligo, cell-type-specific genetic knockouts and engraftment experiments, we find that among multiple interferon-γ (IFNγ)-responsive cell types in vitiligo-affected skin, dermal fibroblasts are uniquely required to recruit and activate CD8 + cytotoxic T cells through secreted chemokines. Anatomically distinct human dermal fibroblasts exhibit intrinsic differences in the expression of chemokines in response to IFNγ. In mouse models of vitiligo, regional IFNγ-resistant fibroblasts determine the autoimmune pattern of depigmentation in the skin. Our study identifies anatomically distinct fibroblasts with permissive or repressive IFNγ responses as the key determinant of body-level patterns of lesions in vitiligo, and highlights mesenchymal subpopulations as therapeutic targets for treating autoimmune diseases. Single-cell analyses of skin samples from patients with vitiligo and functional genetic experiments in vitiligo mouse models show that distinct fibroblast subsets drive the organ level lesion patterns in this autoimmune disease.