Highly stable gold nanoparticles green-synthesized by upcycling cartilage waste extract from yellow-nose skate (Dipturus chilensis) and evaluation of its cytotoxicity, haemocompatibility and antioxidant activity

• Published in: Artificial cells, nanomedicine, and biotechnology Vol. 46; no. sup2; pp. 1108 - 1119
• Main Authors: Ahn, Eun-Young, Lee, You Jeong, Choi, Seo Young, Im, A-Rang, Kim, Yeong Shik, Park, Youmie
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 01.01.2018; Taylor & Francis Ltd
• Subjects: Adenocarcinoma; Anticancer properties; antioxidant activity; Antioxidants; Biocompatibility; Cancer; Cartilage; Cervix; Cubic lattice; Cytotoxicity; Diffraction patterns; Dipturus chilensis; Drug delivery; Drug delivery systems; Gold; Gold nanoparticles; haemocompatibility; Nanoparticles; Nanotechnology; Nose; Reducing agents; Scavenging; skate cartilage wastes; Surface plasmon resonance; Synthesis; Toxicity; Transmission electron microscopy; Wine; X-ray diffraction; skate cartilage wastes; haemocompatibility; antioxidant activity; Gold nanoparticles; cytotoxicity
• ISSN: 2169-1401; 2169-141X
• DOI: 10.1080/21691401.2018.1479710

Skate (Dipturus chilensis) cartilage extract was utilized as a green reducing agent for the synthesis of spherical gold nanoparticles with an average size of 16.7 ± 0.2 nm. The gold nanoparticle solution showed a surface plasmon resonance at 543 nm with a wine-red colour. A strong X-ray diffraction pattern and clear lattice structure in high-resolution transmission electron microscopy indicated a face-centred cubic structure of the gold nanoparticles. The gold nanoparticles retained excellent colloidal stability. Gold nanoparticles showed strong antioxidant activity in terms of 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity. In vitro cytotoxicity was observed for seven cancer cells assessed by the water-soluble tetrazolium assay. Among the seven cancer cells, the highest cytotoxicity was observed for MDA-MB-231 (human breast adenocarcinoma cell) followed by HeLa (human epithelial cervix adenocarcinoma cell) and lastly by HT-29 (human colorectal adenocarcinoma cell). Furthermore, gold nanoparticles showed excellent haemocompatibility, indicating the possibility of their use as a future nanomedicine. These results strongly suggest that gold nanoparticles green-synthesized by upcycling skate cartilage waste extract will be valuable carriers or vehicles for the delivery of drugs or bioactive molecules, such as anti-cancer agents, for the treatment of cancers.