Routine screening for potential babesicides using cultures of Babesia bovis

• Published in: International journal for parasitology Vol. 20; no. 6; pp. 797 - 802
• Main Authors: Nott, Susan E., O'Sulllvan, William J., Gero, Annette M., Bagnara, Aldo S.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.10.1990; Elsevier Science
• Subjects: [ 3H]hypoxanthine incorporation; amicarbalide; analogs; Animals; anti-malarial agents; Antibiotics. Antiinfectious agents. Antiparasitic agents; antimalarials; Antiparasitic agents; antiprotozoal agents; Antiprotozoal Agents - chemistry; Antiprotozoal Agents - pharmacology; Babesia - drug effects; Babesia bovis; Biological and medical sciences; Cattle; culture; hypoxanthine; imidocarb; in vitro; Medical sciences; menoctone; metabolism; Molecular Structure; nucleic acids; nucleosides; Pharmacology. Drug treatments; phlorizin; purine and pyrimidine antagonists; screening; screening in vitro; tetracycline; tubercidin; phlorizin; screening in vitro; anti-malarial agents; menoctone; [ 3H]hypoxanthine incorporation; tetracycline; Babesia bovis; purine and pyrimidine antagonists; Protozoa; Antiprotozoal agent; Efficiency; Parasiticid; Sporozoa; In vitro
• ISSN: 0020-7519; 1879-0135
• DOI: 10.1016/0020-7519(90)90014-E

Routine screening for potential babesicides using cultures of Babesia bovis. International Journal for Parasitology 20: 797–802. A procedure for screening of potential anti-malarial agents, based on the incorporation of [ 3H]hypoxanthine by the parasite, was adapted for the testing of anti-rnetabolites against Babesia bovis (Lismore and Samford isolates) cultured in vitro. A close correlation was found between [ 3H]hypoxanthine incorporation in a standard assay and percentage of parasitized cells as determined by microscopic examination. The concentrations of compounds causing 50% inhibition of [ 3H]hypoxanthine incorporation (ID 50 values) for the established babesicides, Imidocarb and Amicarbalide, were determined to be 3 ng ml −1 (8.6 n m) and 5–10 ng ml −1 (17–34 n m), respectively. A variety of other anti-metabolites were tested in the system. ID 50values for some of the more effective compounds were tubercidin (75 n m), tetracycline (25 μm), menoctone (100 n m) and TN 108, a di-Mannich base derived from 4-(7'-trifluoromethyl-quinolin-4'-ylamino)phenol (0.13 μ m). No significant differences between results with the two isolates of B. bovis were observed.