A Fluorescence Inner-Filter Effect Based Sensing Platform for Turn-On Detection of Glutathione in Human Serum

• Published in: Sensors (Basel, Switzerland) Vol. 19; no. 2; p. 228
• Main Authors: Tang, Shurong, You, Xiuhua, Fang, Quanhui, Li, Xin, Li, Guangwen, Chen, Jinghua, Chen, Wei
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI 09.01.2019; MDPI AG
• Subjects: fluorescence inner-filter effect; glutathione detection; human serum; manganese dioxide nanosheets; molybdenum disulfide quantum dots; fluorescence inner-filter effect; glutathione detection; manganese dioxide nanosheets; molybdenum disulfide quantum dots; human serum
• ISSN: 1424-8220; 1424-8220
• DOI: 10.3390/s19020228

A novel turn-on fluorescence assay was developed for the rapid detection of glutathione (GSH) based on the inner-filter effect (IFE) and redox reaction. Molybdenum disulfide quantum dots (MoS₂ QDs), which have stable fluorescent properties, were synthesized with hydrothermal method. Manganese dioxide nanosheets (MnO₂ NSs) were prepared by exfoliating the bulk δ-MnO₂ material in bovine serum albumin (BSA) aqueous solution. The morphology structures of the prepared nanoparticles were characterized by transmission electron microscope (TEM). Studies have shown that the fluorescence of MoS₂ QDs could be quenched in the presence of MnO₂ NSs as a result of the IFE, and is recovered after the addition of GSH to dissolve the MnO₂ NSs. The fluorescence intensity showed a good linear relationship with the GSH concentration in the range 20⁻2500 μM, the limit of detection was 1.0 μM. The detection method was applied to the analysis of GSH in human serum samples. This simple, rapid, and cost-effective method has great potential in analyzing GSH and in disease diagnosis.